硒和维生素E对甲基汞毒性保护作用的细胞聚集模型

A cell aggregation model for the protective effect of selenium and vitamin E on methylmercury toxicity.

作者信息

Kleinschuster S J, Yoneyama M, Sharma R P

出版信息

Toxicology. 1983 Jan;26(1):1-9. doi: 10.1016/0300-483x(83)90051-3.

Abstract

Histotypic aggregation of embryonic neural retinal cells was chosen as a test model to evaluate mercury toxicity. After 24 h rotational culture with methylmercury (CH3HgCl) at 4 microM, aggregation was completely inhibited. A dose-response relationship between concentrations of methylmercury and final sizes of aggregates was found. Selenium (Na2SeO3) at concentrations of 1, 3 and 5 microM provided a protective effect for methylmercury (1 microM) toxicity. Vitamin E (DL-alpha-Tocopherol acetate) at concentrations 5, 7 and 10 microM also provided protection against the same concentration of methylmercury; however, it was less effective than selenium. Histotypic embryonal retinal cell aggregation may be a useful assay system for in vitro neurotoxic studies in morphogenesis.

摘要

选择胚胎神经视网膜细胞的组织型聚集作为测试模型来评估汞毒性。在4微摩尔的甲基汞（CH3HgCl）中进行24小时旋转培养后，聚集被完全抑制。发现了甲基汞浓度与聚集体最终大小之间的剂量反应关系。浓度为1、3和5微摩尔的硒（Na2SeO3）对甲基汞（1微摩尔）毒性具有保护作用。浓度为5、7和10微摩尔的维生素E（DL-α-生育酚乙酸酯）也对相同浓度的甲基汞提供保护；然而，其效果不如硒。组织型胚胎视网膜细胞聚集可能是形态发生体外神经毒性研究的有用检测系统。