Institute for Neuropathology, Medical Faculty, RWTH Aachen University, Aachen, Germany.
Biomaterials. 2010 Oct;31(30):7705-15. doi: 10.1016/j.biomaterials.2010.06.060. Epub 2010 Jul 24.
In the present in vitro study, the axon growth promoting effects of human neural progenitor-derived astrocytes (hNP-AC) were investigated in simple 2D- as well as in more complex 3D-culture systems. The interactions of the hNP-AC with migrating Schwann cells and fibroblasts were also studied. hNP-AC were found to promote extensive dorsal root ganglion axon regeneration in 2D cultures, being even greater than that observed on the positive control, laminin-coated substrate. Contact-mediated mechanisms and the release of substances into the medium both played a role in supporting axon regeneration. Following seeding onto 3D collagen scaffolds, hNP-AC also promoted significantly greater axon regeneration from DRG explants than was seen on non-seeded scaffolds. The highly orientated, porous microstructure of the scaffold also supported substantial intermixing of hNP-AC and migrating Schwann cells/fibroblasts from the DRG explant, cell populations that are normally mutually repulsive. This suggests that the topography of 3D scaffolds may not only influence cell-substrate interactions but also cell-cell interactions within the scaffold. This opens the possibility that the design of future scaffolds could be optimised to enhance cell integration as well as modulating complex cell-cell interactions.
在本体外研究中，研究了人神经祖细胞来源的星形胶质细胞（hNP-AC）在简单的 2D 以及更复杂的 3D 培养系统中的轴突生长促进作用。还研究了 hNP-AC 与迁移 Schwann 细胞和成纤维细胞的相互作用。研究发现，hNP-AC 在 2D 培养中促进了广泛的背根神经节轴突再生，甚至比在阳性对照物，即层粘连蛋白涂覆的基底上观察到的再生还要多。接触介导的机制和物质在培养基中的释放都在支持轴突再生中发挥了作用。在接种到 3D 胶原支架上后，hNP-AC 还促进了 DRG 外植体的轴突再生，明显大于非接种支架上的再生。支架高度定向的多孔微结构还支持来自 DRG 外植体的 hNP-AC 和迁移 Schwann 细胞/成纤维细胞的大量混合，这些细胞群体通常是相互排斥的。这表明 3D 支架的拓扑结构不仅可以影响细胞-基底相互作用，还可以影响支架内的细胞-细胞相互作用。这为未来支架的设计提供了可能性，可以优化以增强细胞整合，并调节复杂的细胞-细胞相互作用。
