Kim Bum-Soo, Kim Young-Kyun, Yun Pil-Young, Lee Eunha, Bae Jihyun
Department of Oral and Maxillofacial Surgery, Seoul National University Bundang Hospital, Gumi-dong, Bundang-gu, South Korea.
J Oral Maxillofac Surg. 2010 Dec;68(12):2975-9. doi: 10.1016/j.joms.2010.02.023. Epub 2010 Jul 24.
The aim of this study is to identify any association between variants of the polymorphic estrogen receptor gene and various symptoms of temporomandibular disorder (TMD) including pain in the temporomandibular joint and masticatory muscles, joint crepitus, limited range of jaw movement, and bone changes in the condylar head.
Seventy-four patients with TMD were selected according to the Research Diagnostic Criteria for TMD for the study group. Sixty-four patients without TMD were selected as the control group. Genomic DNA was extracted from the epithelial layer of buccal mucosa. After amplification by polymerase chain reaction, direct haplotyping was undertaken to study the restriction fragment length polymorphism of PvuII and XbaI for the α estrogen receptor. Genomic prevalences in each of the symptom categories were analyzed by use of the χ(2) test.
The haplotypes PX, Px, and px constituted 23.0%, 18.9%, and 58.1%, respectively, of the total α estrogen receptor alleles in the study group. The haplotype Px was found to be relatively more prevalent in subjects who had mouth opening limitation, and the haplotype PX was more prevalent in those patients with condylar head bone changes. However, neither of these observations carried statistical significance.
Although certain symptoms of TMD were found to have a relatively higher prevalence of one form or another of the estrogen receptor allele, no haplotype was confirmed to be a significant marker of TMD risk.
本研究旨在确定多态性雌激素受体基因的变体与颞下颌关节紊乱病（TMD）的各种症状之间是否存在关联，这些症状包括颞下颌关节和咀嚼肌疼痛、关节弹响、下颌运动范围受限以及髁突头部的骨质改变。
根据TMD研究诊断标准选择74例TMD患者作为研究组。选择64例无TMD的患者作为对照组。从颊黏膜上皮层提取基因组DNA。通过聚合酶链反应扩增后，进行直接单倍型分析以研究α雌激素受体的PvuII和XbaI限制性片段长度多态性。使用χ²检验分析各症状类别中的基因频率。
在研究组中，单倍型PX、Px和px分别占α雌激素受体等位基因总数的23.0%、18.9%和58.1%。发现单倍型Px在张口受限的受试者中相对更常见，而单倍型PX在髁突头部骨质改变的患者中更常见。然而，这些观察结果均无统计学意义。
尽管发现TMD的某些症状在雌激素受体等位基因的某种形式中具有相对较高的发生率，但没有单倍型被确认为TMD风险的显著标志物。
