Division of Animal Genomics and Bioinformatics, National Institute of Animal science, Rural Development Administration, Suwon, 441-706, Republic of Korea.
Comp Biochem Physiol Part D Genomics Proteomics. 2010 Sep;5(3):234-44. doi: 10.1016/j.cbd.2010.06.004. Epub 2010 Jul 6.
Given the substantial rise in obesity, depot-specific fat accumulation and its associated diseases like diabetes, it is important to understand the molecular basis of depot-specific adipocyte differentiation. Many studies have successfully exploited the adipocyte differentiation, but most of them were not related to depot-specificity, particularly using freshly isolated primary preadipocytes. Using 2-dimensional polyacrylamide gel electrophoresis coupled with sequencing mass spectrometry, we searched and compared the proteins differentially expressed in undifferentiated and differentiated preadipocytes from bovine omental, subcutaneous and intramuscular adipose depots. Our proteome mapping strategy to identify differentially expressed intracellular proteins during adipogenic conversion revealed 65 different proteins that were found to be common for the three depots. Further, we validated the differential expression for a subset of proteins by immunoblotting analyses. The results demonstrated that many structural proteins were down-regulated during differentiation of preadipocytes from all the depots. Most up-regulated proteins like Ubiquinol-cytochrome-c reductase complex core protein I (UQCRC1), ATP synthase D chain, Superoxide dismutase (SOD), Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), Sulfotransferase 1A1 (SULT1A1), Carnitine O-palmitoyltransferase 2 (CPT2) and Heat-shock protein beta 1 (HSPB1) across the three depots were found to be associated with lipid metabolism and metabolic activity. Further, all the up-regulated proteins were found to have higher protein expression in omental than subcutaneous or intramuscular depots.
鉴于肥胖症的大幅上升，脂肪库特异性脂肪堆积及其相关疾病（如糖尿病），了解脂肪细胞分化的脂肪库特异性分子基础非常重要。许多研究已经成功地利用了脂肪细胞分化，但其中大多数与脂肪库特异性无关，特别是使用新分离的原代前体脂肪细胞。我们使用二维聚丙烯酰胺凝胶电泳结合测序质谱，搜索并比较了牛网膜、皮下和肌肉内脂肪库中未分化和分化前体脂肪细胞中差异表达的蛋白质。我们的蛋白质组图谱策略旨在鉴定脂肪生成转化过程中差异表达的细胞内蛋白质，发现了 65 种不同的蛋白质，这些蛋白质在三个脂肪库中是共同的。此外，我们通过免疫印迹分析验证了一组蛋白质的差异表达。结果表明，许多结构蛋白在前体脂肪细胞分化过程中下调。大多数上调的蛋白质，如泛醌-细胞色素-c 还原酶复合物核心蛋白 I（UQCRC1）、ATP 合酶 D 链、超氧化物歧化酶（SOD）、甘油醛-3-磷酸脱氢酶（GAPDH）、磺基转移酶 1A1（SULT1A1）、肉碱 O-棕榈酰基转移酶 2（CPT2）和热休克蛋白β 1（HSPB1）在三个脂肪库中都与脂质代谢和代谢活性有关。此外，所有上调的蛋白质在前膜中的蛋白表达都高于皮下或肌肉内脂肪库。
