Schuchert P, Langsford M, Käslin E, Kohli J
Institute of General Microbiology, University of Bern, Switzerland.
EMBO J. 1991 Aug;10(8):2157-63. doi: 10.1002/j.1460-2075.1991.tb07750.x.
The point mutation M26 in the ade6 gene of Schizosaccharomyces pombe increases recombination frequency by an order of magnitude in comparison with other mutations in the same gene. The hypothesis is tested that this hot spot of recombination requires a specific nucleotide sequence at the M26 site. The DNA sequence is altered systematically by in vitro mutagenesis, and the resulting sequences are introduced into the ade6 gene in vivo by gene replacement. It results that any change of the heptanucleotide ATGACGT leads to loss of high frequency of recombination. Thus this oligonucleotide sequence is necessary for high frequency of recombination, but it seems not to be sufficient.
粟酒裂殖酵母ade6基因中的点突变M26与同一基因中的其他突变相比，使重组频率提高了一个数量级。对这一假设进行了检验，即这种重组热点在M26位点需要特定的核苷酸序列。通过体外诱变系统地改变DNA序列，并通过基因替换将所得序列体内导入ade6基因。结果表明，七核苷酸ATGACGT的任何变化都会导致高频重组的丧失。因此，这种寡核苷酸序列对于高频重组是必要的，但似乎并不充分。
