Bibi Fehmida, Chung Eu Jin, Jeon Che Ok, Chung Young Ryun
Division of Applied Life Science (BK 21), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju 660-701, Republic of Korea.
JGreen Inc., Department of Research & Development, Jinju 660-841, Republic of Korea.
Int J Syst Evol Microbiol. 2011 Jul;61(Pt 7):1567-1571. doi: 10.1099/ijs.0.023820-0. Epub 2010 Jul 23.
A Gram-stain-positive endophytic bacterium, designated strain YC6957(T), was isolated from surface-sterilized roots of a halophyte (Elymus mollis Trin.) inhabiting coastal tidal flats of Namhae Island, located on the southern coast of Korea, and was subjected to a polyphasic taxonomic study. Cells were facultatively anaerobic, endospore-forming rods to coccoid rods, motile by a single flagellum. Strain YC6957(T) was catalase-positive, oxidase-negative and able to grow in the presence of 0-8 % (w/v) NaCl, with optimum growth at 4-5 % (w/v) NaCl. Growth occurred at 15-45 °C (optimal growth at 30-35 °C) and pH 6.0-8.5 (optimal growth at pH 7.0-8.0). The predominant isoprenoid quinone was menaquinone 7 (MK-7). The major cellular fatty acids were C(16 : 0) (11.3 %), iso-C(15 : 0) (19.2 %) and anteiso-C(15 : 0) (36.4 %). The cell-wall peptidoglycan contained meso-diaminopimelic acid. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The DNA G+C content was 41.6 mol%. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that the isolate belonged to the genus Bacillus. Strain YC6957(T) exhibited high 16S rRNA gene sequence similarity to its closest neighbours, Bacillus ruris LMG 22866(T) (96.14 %), Bacillus lentus NCIMB 8773(T) (95.97 %) and Bacillus galactosidilyticus LMG 17892(T) (95.91 %), and less than 95.84 % similarity to all other type strains in the genus Bacillus. On the basis of the phylogenetic, physiological and biochemical data, it is suggested that strain YC6957(T) represents a novel species of the genus Bacillus, for which the name Bacillus graminis sp. nov. is proposed. The type strain is YC6957(T) ( = KACC 13779(T) = DSM 22162(T)).
从韩国南海岸南海岛潮间带的盐生植物（软穗披碱草）表面消毒的根部分离出一株革兰氏阳性内生细菌，命名为菌株YC6957(T)，并对其进行了多相分类研究。细胞为兼性厌氧，形成芽孢的杆状至球状杆状，具单根鞭毛运动。菌株YC6957(T)过氧化氢酶阳性，氧化酶阴性，能在0-8%（w/v）NaCl存在下生长，最适生长NaCl浓度为4-5%（w/v）。在15-45℃（最适生长温度30-35℃）和pH 6.0-8.5（最适生长pH 7.0-8.0）条件下生长。主要类异戊二烯醌为甲基萘醌7（MK-7）。主要细胞脂肪酸为C(16 : 0)（11.3%）、异-C(15 : 0)（19.2%）和anteiso-C(15 : 0)（36.4%）。细胞壁肽聚糖含有内消旋二氨基庚二酸。主要极性脂为双磷脂酰甘油、磷脂酰甘油和磷脂酰乙醇胺。DNA G+C含量为41.6 mol%。基于16S rRNA基因序列的系统发育分析表明，该分离株属于芽孢杆菌属。菌株YC6957(T)与其最接近的亲缘种鲁氏芽孢杆菌LMG 22866(T)（96.14%）、迟缓芽孢杆菌NCIMB 8773(T)（95.97%）和半乳糖分解芽孢杆菌LMG 17892(T)（95.91%）表现出较高的16S rRNA基因序列相似性，与芽孢杆菌属的所有其他模式菌株相似性均低于95.84%。根据系统发育、生理和生化数据，建议菌株YC6957(T)代表芽孢杆菌属的一个新种，命名为禾本科芽孢杆菌（Bacillus graminis sp. nov.）。模式菌株为YC6957(T)（=KACC 13779(T)=DSM 22162(T)）。
