Liu Yan, Yu Min, Zhang Xiao-Hua
College of Marine Life Sciences, Ocean University of China, Qingdao 266003, PR China.
Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, PR China.
Int J Syst Evol Microbiol. 2018 Apr;68(4):1184-1189. doi: 10.1099/ijsem.0.002648. Epub 2018 Feb 21.
A Gram-stain-positive, endospore-forming, rod-shaped, motile and strictly aerobic strain, designated T3-209, was isolated from sediment sampled at a water depth of 1206 m of the southern Okinawa Trough at station T1 (25.07° N, 122.58° E) near the Tangyin hydrothermal vent during an expedition on the R/V Kexue in May 2014. The strain was able to grow at a temperature range of 10-42 °C (optimum 28 °C). Growth was observed at NaCl concentrations (w/v) of 0-6 % (optimum 0 %). The pH range for growth was 7.0-9.0 (optimum 8.0). The 16S rRNA gene sequence analysis indicated that strain T3-209 belonged to the genus Bacillus within the family Bacillaceae and was most closely related to Bacillus drentensis LMG 21831 and Bacillus bataviensis LMG 21833, with the same sequence similarity (97.0 %), but shared relatively low levels of similarity (92.6-96.9 %) to the type strains of other Bacillus species. The peptidoglycan type of strain T3-209 was meso-diaminopimelic acid. The predominant cellular fatty acids were anteiso-C15 : 0, iso-C15 : 0 and alcohol-C16 : 1ω7c. The respiratory quinone was menaquinone-7. The polar lipids of strain T3-209 comprised phosphatidylglycerol, two unidentified phospholipids and one unidentified aminolipid. The DNA G+C content was 41.8 mol%. Combining results of phylogenetic analysis, phenotypic characterization and chemotaxonomic studies, strain T3-209 represents a novel species of the genus Bacillus, for which the name Bacillus alkalitolerans sp. nov. is proposed. The type strain is T3-209 (=KCTC 33631=DSM 29135=MCCC 1K00503).
2014年5月，在“科学”号科考船上的一次考察中，从冲绳海槽南部水深1206米、T1站（北纬25.07°，东经122.58°）靠近汤阴热液喷口处采集的沉积物中，分离出一株革兰氏染色阳性、形成芽孢、杆状、具运动性且严格需氧的菌株，命名为T3-209。该菌株能够在10-42°C的温度范围内生长（最适温度28°C）。在NaCl浓度（w/v）为0-6%（最适浓度0%）时可观察到生长。生长的pH范围为7.0-9.0（最适pH 8.0）。16S rRNA基因序列分析表明，菌株T3-209属于芽孢杆菌科芽孢杆菌属，与德伦特芽孢杆菌LMG 21831和巴达维亚芽孢杆菌LMG 21833关系最为密切，序列相似性相同（97.0%），但与其他芽孢杆菌属模式菌株的相似性相对较低（92.6-96.9%）。菌株T3-209的肽聚糖类型为内消旋二氨基庚二酸。主要的细胞脂肪酸为anteiso-C15 : 0、iso-C15 : 0和alcohol-C16 : 1ω7c。呼吸醌为甲基萘醌-7。菌株T3-209的极性脂质包括磷脂酰甘油、两种未鉴定的磷脂和一种未鉴定的氨基脂质。DNA G+C含量为41.8 mol%。综合系统发育分析、表型特征和化学分类学研究结果，菌株T3-209代表芽孢杆菌属的一个新物种，为此提出新名称嗜碱芽孢杆菌（Bacillus alkalitolerans sp. nov.）。模式菌株为T3-209（=KCTC 33631=DSM 29135=MCCC 1K00503）。
