采用β-rec/six 位点特异性重组系统对人类病原体烟曲霉中的自我切除标记进行验证。
Validation of a self-excising marker in the human pathogen Aspergillus fumigatus by employing the beta-rec/six site-specific recombination system.
机构信息
Research Center for Infectious Diseases, Julius-Maximilians-University Würzburg, D-97080 Würzburg, Germany.
出版信息
Appl Environ Microbiol. 2010 Sep;76(18):6313-7. doi: 10.1128/AEM.00882-10. Epub 2010 Jul 23.
Abstract
Recyclable markers based on site-specific recombination allow repetitive gene targeting in filamentous fungi. Here we describe for the first time functionality of the bacterial recombination system employing beta serine recombinase acting on six recognition sequences (beta-rec/six) in a fungal host, the human pathogen Aspergillus fumigatus, and its use in establishing a self-excising resistance marker cassette for serial gene replacement.
摘要
基于位点特异性重组的可回收标记物允许丝状真菌中重复的基因靶向。在这里，我们首次描述了在真菌宿主、人类病原体烟曲霉中利用β丝氨酸重组酶作用于六个识别序列（β-rec/six）的细菌重组系统的功能，以及其在建立用于连续基因替换的自我切除抗性标记盒中的应用。
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