Mai M D, Sahana G, Christiansen F B, Guldbrandtsen B
Department of Biology, Research Institute for Aquaculture No. 3, Dangtat, Vinhhai, Nhatrang, Vietnam.
J Anim Sci. 2010 Nov;88(11):3522-8. doi: 10.2527/jas.2009-2713. Epub 2010 Jul 23.
Quantitative trait loci for milk production traits in Danish Jersey cattle were mapped by a genome-wide association analysis using a mixed model. The analysis incorporated 1,039 bulls and 33,090 SNP and resulted in 98 detected combinations of QTL and traits on 27 BTA. These QTL comprised 30 for milk index, 50 for fat index, and 18 for protein index. The evidence presents 33 genome-wide QTL on 14 BTA. Of these, 7 had effects on milk index, 21 on fat index, and 5 on protein index. Among the genome-wide QTL, 26 have been previously reported, 2 on BTA4 and BTA5 were new for milk index, and 5 on BTA4, BTA5, BTA13, BTA20, and BTA29 were new QTL for fat index. We found 7 pleiotropic or very closely linked QTL. Most of the QTL were associated with polymorphisms within narrow regions and several may represent the effects of polymorphisms of genes: DGAT1, casein, ARFGAP3, CYP11B1, and CDC-like kinase 4. By a chromosome-wide threshold, 65 additional QTL were detected. Many of them are likely to represent QTL. The results are interesting from a breeding perspective and contribute to the search for the genes causing the polymorphisms important for milk production traits.
利用混合模型通过全基因组关联分析对丹麦泽西牛产奶性状的数量性状位点进行了定位。该分析纳入了1039头公牛和33090个单核苷酸多态性（SNP），并在27条牛染色体（BTA）上检测到98个数量性状位点（QTL）与性状的组合。这些QTL包括30个影响产奶指数的、50个影响脂肪指数的和18个影响蛋白质指数的。证据显示在14条BTA上有33个全基因组QTL。其中，7个对产奶指数有影响，21个对脂肪指数有影响，5个对蛋白质指数有影响。在全基因组QTL中，26个先前已有报道，BTA4和BTA5上有2个产奶指数的新QTL，BTA4、BTA5、BTA13、BTA20和BTA29上有5个脂肪指数的新QTL。我们发现了7个多效性或紧密连锁的QTL。大多数QTL与狭窄区域内的多态性相关，有几个可能代表了基因多态性的影响：二酰基甘油酰基转移酶1（DGAT1）、酪蛋白、ADP核糖基化因子GTP酶激活蛋白3（ARFGAP3）、细胞色素P450 11B1（CYP11B1）和类细胞周期蛋白依赖性激酶4（CDC-like kinase 4）。通过染色体范围的阈值，又检测到65个额外的QTL。其中许多可能代表QTL。从育种角度来看，这些结果很有趣，有助于寻找导致对产奶性状重要的多态性的基因。
