利用固态纳米孔检测 DNA 杂交。
Detection of DNA hybridizations using solid-state nanopores.
机构信息
Department of Physics, Brown University, Providence, RI 02912, USA.
出版信息
Nanotechnology. 2010 Aug 20;21(33):335102. doi: 10.1088/0957-4484/21/33/335102. Epub 2010 Jul 26.
Abstract
We report an experimental study of using DNA translocation through solid-state nanopores to detect the sequential arrangement of two double-stranded 12-mer hybridization segments on a single-stranded DNA molecule. The sample DNA is a trimer molecule formed by hybridizing three single-stranded oligonucleotides. A polystyrene bead is attached to the end of the trimer DNA, providing a mechanism in slowing down the translocation and suppressing the thermal diffusion, thereby allowing the detection of short features of DNA by standard patch-clamp electronics. The electrical signature of the translocation of a trimer molecule through a nanopore has been identified successfully in the temporal traces of ionic current. The results reported here represent the first successful attempt in using a solid-state nanopore as an ionic scanning device in resolving individual hybridization segments (or 'probes') on a DNA molecule.
摘要
我们报告了一项实验研究，即通过固态纳米孔中的 DNA 迁移来检测单链 DNA 分子上两个双链 12 聚体杂交片段的顺序排列。该样本 DNA 是由三个单链寡核苷酸杂交形成的三聚体分子。三聚体 DNA 的末端连接一个聚苯乙烯珠，提供了一种减缓迁移和抑制热扩散的机制，从而允许通过标准的膜片钳电子设备检测 DNA 的短特征。在离子电流的时间轨迹中，成功地识别出三聚体分子通过纳米孔迁移的电信号特征。这里报道的结果代表了首次成功尝试将固态纳米孔用作离子扫描设备，以解析 DNA 分子上的单个杂交片段（或“探针”）。
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