Department of Biomaterials Science, Graduate School of Biomedical Sciences, Hiroshima University, Minami-ku, Hiroshima, Japan.
Dent Mater J. 2010 Aug;29(4):438-45. doi: 10.4012/dmj.2010-017. Epub 2010 Jul 17.
Hydroxyapatite formation was examined at the surface of self-assembled monolayers (SAMs) modified with four functional groups, -COOH, -NH(2), -CH(3), and -OH. For COOH-SAM and NH(2)-SAM, scanning electron spectroscopic observation showed that flake-like sheet crystals covered the whole wafer and small broccoli-like crystals were observed occasionally on the flake-like crystal base layer. For CH(3)-SAM and OH-SAM, no flake-like sheet crystals were observed; broccoli-like crystals were observed in a dispersed manner for CH(3)-SAM, but in localized spots for OH-SAM. X-ray diffraction patterns showed a strong apatite pattern oriented toward the c-axis direction for COOH-SAM. ESCA analysis revealed distinct Ca, P, O peaks for COOH-, NH(2)-, CH(3)-, and OH-SAM. Surface plasmon resonance (SPR) analysis indicated that during the supply of supersaturated calcium phosphate solution, the deposition of precipitates increased monotonically with time for COOH-SAM, increased slightly for NH(2)-SAM, but little increase in deposition was detected for CH(3)-SAM and OH-SAM.
水合羟基磷灰石的形成在经-COOH、-NH2、-CH3 和-OH 四种官能团修饰的自组装单分子层（SAM）表面进行了检测。对于 COOH-SAM 和 NH2-SAM，扫描电子光谱观察表明片状晶体覆盖了整个晶片，并且偶尔在片状晶体基底上观察到小的花椰菜状晶体。对于 CH3-SAM 和 OH-SAM，未观察到片状晶体；CH3-SAM 中的花椰菜状晶体呈分散状，而 OH-SAM 中的花椰菜状晶体呈局域状。X 射线衍射图谱显示 COOH-SAM 具有强烈的沿 c 轴方向的磷灰石图案。ESCA 分析表明，COOH-、NH2-、CH3- 和 OH-SAM 均具有明显的 Ca、P、O 峰。表面等离子体共振（SPR）分析表明，在供应过饱和磷酸钙溶液期间，对于 COOH-SAM，沉淀物的沉积随时间单调增加，对于 NH2-SAM，略有增加，但对于 CH3-SAM 和 OH-SAM，沉积增加很少。
