Department of Chemistry, Faculty of Sciences, Trg Dositeja Obradovica 3, 21000 Novi Sad, Serbia.
Molecules. 2010 Jun 24;15(7):4564-71. doi: 10.3390/molecules15074564.
In this study the effects of an aqueous suspension of a commercial preparation of the mushroom Coprinus comatus on oxidative stress induced in rats by alloxane and carbon tetrachloride was examined. The effects were estimated from changes in the biochemical parameters (xanthine oxidase, glutathione peroxidase and catalase activity, reduced glutathione content, and extent of lipid peroxidation) of liver homogenate as well as histological changes in the liver of the rats treated with alloxane and carbon tetrachloride. Two screening doses of alloxane sufficient to induce diabetes in rats did not have any significant effect on the examined biochemical parameters of liver homogenate or on the cytoarchitectonics of liver cross-sections. Treatment with carbon tetrachloride resulted in a significant increase in the intensity of lipid peroxidation and peroxydasis activity, as well as with decrease in catalase activity. Certain changes in liver cross sections were detected, such is lymphocyte infiltration of dilated sinusoid capillaries. Administration of Coprinus comatus suspension thus showed antioxidative potential, evidenced by an increase of antioxidative status of liver homogenate and prevention of histological changes in liver cross sections.
在这项研究中，检查了蘑菇 Coprinus comatus 的商业制剂的水混悬液对丙烯醛和四氯化碳诱导的大鼠氧化应激的影响。从肝匀浆的生化参数（黄嘌呤氧化酶、谷胱甘肽过氧化物酶和过氧化氢酶活性、还原型谷胱甘肽含量以及脂质过氧化程度）的变化以及用丙烯醛和四氯化碳处理的大鼠肝组织学变化来估计这些影响。两个足以诱导大鼠糖尿病的丙烯醛筛选剂量对肝匀浆的检查生化参数或肝切片的细胞结构没有任何显著影响。四氯化碳处理导致脂质过氧化和过氧化物酶活性显著增加，过氧化氢酶活性降低。还检测到肝切片的某些变化，例如淋巴细胞浸润扩张的窦状毛细血管。因此，Coprinus comatus 混悬液的给药显示出抗氧化潜力，这表现在肝匀浆的抗氧化状态增加和防止肝切片的组织学变化。
