Key Laboratory of Delivery Systems of Chinese Meteria Medica, Jiangsu Provincial Academy of Chinese Medicine, Jiangsu, Nanjing, 210028, China.
Molecules. 2010 Jul 16;15(7):4934-46. doi: 10.3390/molecules15074934.
The antioxidant activities of the various extracts and fractions from the herbs of Artemisia selegensis Turcz (AST) were investigated by in vitro and in vivo assays. FRAP, DPPH and ABTS assays were used to evaluate the antioxidant activities of the extracts/fractions in vitro. The effect of water extract (WE) in reducing oxidative stress in male mice was evaluated. Phenolic acid compounds contribute significantly to the antioxidant activity. From the results of three in vitro antioxidant assays, WE was found to have the highest antioxidant activity, and among the WE subfractions, the water soluble fraction has a significant antioxidant activity. The in vivo antioxidant assay results showed that high doses of WE significantly decrease the MDA level compared to normal diet and D-(+) galactose group (p < 0.05), and the SOD activity of mice given a high dose of WE was the highest. These in vitro and in vivo studies demonstrated that the extracts, especially the WE from AST, have significant antioxidant and free radical scavenging activities. In summary, we propose that AST could be potentially used as a rich source of natural antioxidants.
采用体外和体内试验研究了黄花蒿（Artemisia selegensis Turcz）不同提取物和馏分的抗氧化活性。采用 FRAP、DPPH 和 ABTS 法评价提取物/馏分的体外抗氧化活性。评价水提取物（WE）对雄性小鼠氧化应激的降低作用。酚酸类化合物对其抗氧化活性有显著贡献。从三种体外抗氧化试验的结果来看，WE 表现出最高的抗氧化活性，而在 WE 的亚组分中，水溶性组分具有显著的抗氧化活性。体内抗氧化试验结果表明，与正常饮食和 D-（+）半乳糖组相比，高剂量 WE 可显著降低 MDA 水平（p < 0.05），且给予高剂量 WE 的小鼠的 SOD 活性最高。这些体外和体内研究表明，提取物，特别是来自黄花蒿的 WE，具有显著的抗氧化和自由基清除活性。综上所述，我们提出黄花蒿可能是天然抗氧化剂的潜在丰富来源。
