Wacker Chemie AG, Analytical Services, Johannes-Hess-Strasse 24, 84489 Burghausen, Germany.
Rapid Commun Mass Spectrom. 2010 Aug 30;24(16):2439-44. doi: 10.1002/rcm.4668.
The principle relating to the selection of a proper matrix, cationization reagent, and solvent for matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) of synthetic polymers is still a topic of research. In this work we focused on the selection of a suitable MALDI solvent. Polystyrene PS7600 and poly(ethylene glycol) PEG4820 were analyzed by MALDI-TOF MS using various solvents which were selected based on the Hansen solubility parameter system. For polystyrene (PS), dithranol was used as the matrix and silver trifluoroacetate as the cationization reagent whereas, for poly(ethylene glycol) (PEG), the combination of 2,5-dihydroxybenzoic acid and sodium trifluoroacetate was used for all experiments. When employing solvents which dissolve PS and PEG, reliable MALDI mass spectra were obtained while samples in non-solvents (solvents which are not able to dissolve the polymer) failed to provide spectra. It seems that the solubility of the matrix and the cationization reagent are less important than the polymer solubility.
对于将合成聚合物的基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF MS）转化为简体中文，选择适当的基质、阳离子化试剂和溶剂的原则仍然是一个研究课题。在这项工作中，我们专注于选择合适的 MALDI 溶剂。使用基于 Hansen 溶解度参数系统选择的各种溶剂，通过 MALDI-TOF MS 分析聚苯乙烯 PS7600 和聚乙二醇 PEG4820。对于聚苯乙烯（PS），使用二羟蒽醇作为基质，并用三氟乙酸银作为阳离子化试剂，而对于聚乙二醇（PEG），在所有实验中都使用 2,5-二羟基苯甲酸和三氟乙酸钠的组合。当使用能够溶解 PS 和 PEG 的溶剂时，获得了可靠的 MALDI 质谱，而在非溶剂（不能溶解聚合物的溶剂）中，样品则无法提供谱图。似乎基质和阳离子化试剂的溶解度不如聚合物的溶解度重要。
