Nersesyan A, Chobanyan N
National Center of Oncology ,Yerevan, Armenia.
J BUON. 2010 Apr-Jun;15(2):337-9.
To evaluate the genetic instability in somatic cells of patients with polycystic ovary syndrome (PCOS) by means of study of micronuclei (MN) level in exfoliated buccal cells and DNA damage in leukocytes.
The levels of MN in exfoliated buccal cells and DNA damage in leukocytes of 17 PCOS patients and 17 healthy women were studied. Except MN, other nuclear anomalies connected both with genotoxicity and cytotoxicity were evaluated. DNA damage was evaluated by means of the comet or singlecell gel electrophoresis assay in leukocytes.
The results of our study showed significantly increased frequencies of MN but not of other nuclear anomalies in exfoliated buccal cells of PCOS patients. DNA in leukocytes was also found significantly damaged compared with healthy females.
Genetic instability can have very serious consequences for PCOS patients because of established correlations of increased levels of MN and chromosomal aberration with cancer incidence. Hence, more scrupulous investigations in this area are certainly warranted.
通过研究脱落颊细胞中的微核（MN）水平和白细胞中的DNA损伤，评估多囊卵巢综合征（PCOS）患者体细胞的遗传不稳定性。
研究了17例PCOS患者和17名健康女性的脱落颊细胞中的MN水平以及白细胞中的DNA损伤。除MN外，还评估了与遗传毒性和细胞毒性相关的其他核异常。通过彗星试验或白细胞中的单细胞凝胶电泳分析评估DNA损伤。
我们的研究结果显示，PCOS患者脱落颊细胞中的MN频率显著增加，但其他核异常未增加。与健康女性相比，还发现白细胞中的DNA受到显著损伤。
由于已确定MN水平升高和染色体畸变与癌症发病率之间的相关性，遗传不稳定性可能对PCOS患者产生非常严重的后果。因此，在该领域进行更严格的研究当然是必要的。
