Department of Medical Biology, Medical Faculty, Erciyes University, Kayseri, Turkey.
Gynecol Endocrinol. 2010 Aug;26(8):590-5. doi: 10.3109/09513591003632142.
Polycystic ovary syndrome (PCOS) has recently been linked with genomic instability and DNA damage. The aim of this study was to test genomic damage in women PCOS, using two different methods for assessing damage in both chromosome and base level. The study was performed on 36 newly diagnosed women with PCOS and 29 healthy women as controls. The micronucleus (MN) analysis used as a biomarker of chromosomal/DNA damage was performed in peripheral lymphocytes by cytokinesis-block method. 8-hydroxydeoxyguanosine (8-OHdG) levels used as a reliable marker of oxidative DNA damage were measured in plasma using an ELISA kit. We found that MN frequencies obtained from lymphocytes of the women with PCOS were significantly higher than those of controls (4.1 +/- 1.0 vs. 2.1 +/- 0.6, P = 0.001), whereas, no differences in 8-OHdG level were found between the patients with PCOS and controls (0.5 +/- 0.3 vs. 0.5 +/- 0.2, P = 0.858). These findings indicate that women with PCOS seem to have increased genomic instability, but do not appear to have oxidative DNA damage despite the increased oxidative stress associated with PCOS.
多囊卵巢综合征（PCOS）最近与基因组不稳定性和 DNA 损伤有关。本研究旨在使用两种不同的方法评估染色体和碱基水平的损伤，以测试 PCOS 女性的基因组损伤。该研究共纳入 36 名新诊断的 PCOS 女性和 29 名健康女性作为对照组。微核（MN）分析作为染色体/DNA 损伤的生物标志物，通过细胞分裂阻断法在周围淋巴细胞中进行。使用 ELISA 试剂盒测量血浆中 8-羟基脱氧鸟苷（8-OHdG）水平作为氧化 DNA 损伤的可靠标志物。我们发现，PCOS 女性的淋巴细胞 MN 频率明显高于对照组（4.1 +/- 1.0 对 2.1 +/- 0.6，P = 0.001），而 PCOS 患者和对照组之间 8-OHdG 水平没有差异（0.5 +/- 0.3 对 0.5 +/- 0.2，P = 0.858）。这些发现表明，尽管与 PCOS 相关的氧化应激增加，但 PCOS 女性似乎存在基因组不稳定性增加，但似乎没有氧化 DNA 损伤。
