Hadzi-Mihailovic M, Raybaud H, Monteil R, Cakic S, Djuric M, Jankovic L
Clinic of Periodontology and Oral Medicine, Faculty of Dentistry, University of Belgrade, Belgrade, Serbia.
J BUON. 2010 Apr-Jun;15(2):362-8.
The aim of this study was to investigate the malignant potential of oral lichen planus (OLP) on the basis of the expression of the Bcl-2 marker in healthy individuals (H), patients with OLP and patients with squamous cell carcinoma (SCC), and to establish potential interdependence between expression of Bcl-2 and the different clinical and histopathological parameters in H, OLP, and SCC.
Immunohistochemistry for Bcl-2 was carried out using an avidin-biotin peroxidase complex method. Tissue sections were treated with mouse monoclonal antibody against Bcl-2 (124, DAKO A/S, Denmark; dilution 1/100). Immunohistochemical parameters measured included total tissue area, total stained area and intensity of stain.
Keratinocytes were not Bcl-2 positive in H and were sparsely positive in OLP. The number of lymphocytes stained with Bcl-2 was significantly lower in H as compared to patients with SCC and OLP. Bcl-2 staining was weak to moderate in OLP, and moderate to intense in SCC. Bcl-2-positive lymphocytes were more expressed in older OLP patients (>55 years), and in OLP specimens with orthokeratinized epithelium, less developed acanthosis and highest grade of lymphocyte expression.
Bcl-2 may not serve as a prognostic biomarker in oral cancer development from OLP, but it could help in selecting patients with higher need of follow up to prevent malignancy.
本研究旨在基于Bcl-2标志物在健康个体（H）、口腔扁平苔藓（OLP）患者和鳞状细胞癌（SCC）患者中的表达情况，探讨口腔扁平苔藓的恶性潜能，并确定H、OLP和SCC中Bcl-2表达与不同临床和组织病理学参数之间的潜在相互关系。
采用抗生物素蛋白-生物素过氧化物酶复合物法进行Bcl-2免疫组织化学检测。组织切片用抗Bcl-2小鼠单克隆抗体（124，丹麦DAKO A/S公司；稀释度1/100）处理。测量的免疫组织化学参数包括总组织面积、总染色面积和染色强度。
H组角质形成细胞Bcl-2呈阴性，OLP组呈散在阳性。与SCC患者和OLP患者相比，H组中Bcl-2染色的淋巴细胞数量显著减少。OLP组Bcl-2染色为弱阳性至中度阳性，SCC组为中度至强阳性。Bcl-2阳性淋巴细胞在年龄较大（>55岁）的OLP患者以及具有正角化上皮、棘层增厚不明显和淋巴细胞表达程度最高的OLP标本中表达更多。
Bcl-2可能不是OLP发展为口腔癌的预后生物标志物，但它有助于选择需要更高随访频率以预防恶变的患者。
