Hadzi-Mihailovic M, Raybaud H, Monteil R, Jankovic L
Clinic of Periodontology and Oral Medicine, Faculty of Dentistry, University of Belgrade, Belgrade, Serbia.
J BUON. 2009 Jul-Sep;14(3):487-93.
To investigate the malignant potential of oral lichen planus (OLP) on the basis of expression of the Fas/FasL markers in healthy individuals (H), OLP patients and patients with squamous cell carcinoma (SCC).
40 patients with OLP and two control groups were included in this research (H and patients with SCC). Immunohistochemistry for Fas and FasL was carried out using an avidin-biotin peroxidase complex method.
Only a low percentage of infiltrating lymphocytes and no keratinocytes were Fas-positive in OLP specimens. The highest percentage of Fas-staining keratinocytes in our survey was identified mostly in H and patients with well-differentiated SCC. In most cases of SCC, OLP and H a high percentage of keratinocytes and lymphocytes were FasL-positive. FasL expression was negatively correlated with the degree of cell differentiation and apoptosis. Taking into consideration that all carcinomas in this survey were highly differentiated, it is not surprising that no statistically significant differences in FasL expression between H, OLP and SCC specimens were detected.
Downregulation of Fas expression in keratinocytes and lymphocytes of OLP specimens, together with upregulation of FasL, may serve as initial prognostic biomarker in oral cancer development.
基于Fas/FasL标志物在健康个体（H）、口腔扁平苔藓（OLP）患者和鳞状细胞癌（SCC）患者中的表达情况，研究口腔扁平苔藓的恶性潜能。
本研究纳入40例OLP患者及两个对照组（H和SCC患者）。采用抗生物素蛋白-生物素过氧化物酶复合物法进行Fas和FasL的免疫组织化学检测。
在OLP标本中，仅有低比例的浸润淋巴细胞Fas阳性，且角质形成细胞均为Fas阴性。在我们的研究中，Fas染色角质形成细胞比例最高的主要见于H组和高分化SCC患者。在大多数SCC、OLP和H组病例中，高比例的角质形成细胞和淋巴细胞FasL阳性。FasL表达与细胞分化程度和凋亡呈负相关。鉴于本研究中的所有癌均为高分化，因此未检测到H组、OLP组和SCC标本之间FasL表达的统计学显著差异也就不足为奇了。
OLP标本中角质形成细胞和淋巴细胞Fas表达下调，同时FasL上调，可能是口腔癌发生发展的初始预后生物标志物。
