Stringer M A, Timberlake W E
Department of Genetics, University of Georgia, Athens 30602, USA.
Mol Microbiol. 1995 Apr;16(1):33-44. doi: 10.1111/j.1365-2958.1995.tb02389.x.
An anonymous cDNA clone, pCAN4, was shown previously to correspond to an mRNA that accumulates preferentially during asexual sporulation of the filamentous fungus Aspergillus nidulans. The peptide encoded by pCAN4 is a fungal hydrophobin, a group of small, hydrophobic cell wall proteins. When the CAN4 gene was disrupted, conidia and conidiophores appeared to be normal, but sporulating colonies wetted more rapidly with detergent solutions than did the wild type. We renamed CAN4 dewA for the detergent wettable phenotype and mapped it to chromosome V, 24 map units from cysC. The A. nidulans rodA gene also encodes a sporulation-specific fungal hydrophobin. Spores of a dewA- rodA- double mutant were less hydrophobic than those of either mutant alone, showing that dewA and rodA contribute independently to spore-wall hydrophobicity. Immunolocalization of DewA by epitope tagging demonstrated that DewA is present in the spore wall, but not in the walls of germ tubes, hyphae or cells of the spore-producing conidiophore. We conclude that dewA encodes a new fungal hydrophobin component of the conidial wall.
先前已证明，一个名为pCAN4的匿名cDNA克隆与一种在丝状真菌构巢曲霉无性孢子形成过程中优先积累的mRNA相对应。pCAN4编码的肽是一种真菌疏水蛋白，即一组小的疏水性细胞壁蛋白。当CAN4基因被破坏时，分生孢子和分生孢子梗看起来正常，但与野生型相比，产孢菌落被洗涤剂溶液浸湿的速度更快。我们将CAN4重新命名为dewA，以体现其对洗涤剂可湿性的表型，并将其定位到V号染色体上，距离cysC基因24个图距单位。构巢曲霉rodA基因也编码一种孢子形成特异性真菌疏水蛋白。dewA - rodA - 双突变体的孢子疏水性低于单独的任一突变体，表明dewA和rodA对孢子壁疏水性的贡献是独立的。通过表位标签对DewA进行免疫定位表明，DewA存在于孢子壁中，但不存在于芽管、菌丝或产孢分生孢子梗的细胞壁中。我们得出结论，dewA编码一种新的分生孢子壁真菌疏水蛋白成分。
