Abbassy Mona A, Watari Ippei, Soma Kunimichi
Orthodontic Science, Department of Orofacial Development and Function, Division of Oral Health Sciences, Graduate School, Tokyo Medical and Dental University, Yushima, Bunkyo-ku, Tokyo, Japan.
Eur J Oral Sci. 2010 Aug;118(4):364-9. doi: 10.1111/j.1600-0722.2010.00739.x.
The aim of this study was to assess the effect of type 1 diabetes mellitus (DM) on the structure of mandibular bone and on the changes of alveolar/jaw bone formation. Experimental DM was induced in 3-wk-old male Wistar rats by a single dose of 60 mg/kg body weight of streptozotocin. All rats were injected with calcein on days 21 and 28. The rats were killed when 8 wk of age. Bone structure was analyzed by bone histomorphometry, microcomputed tomography (micro-CT), and histological section. Histomorphometric analysis showed that the mineral apposition and the bone formation rates in most of the mandibular regions were significantly decreased in the DM group compared with the control group. Micro-CT analysis showed significant deterioration of the bone quality in rats with DM. For a histometric measure of bone resorption, the number of osteoclasts along the distal surface of the alveolar wall was counted. The number of osteoclasts was significantly lower in the rats with DM than in the controls. These findings suggest that uncontrolled DM decreases mandibular bone formation, reduces the rate of bone turnover in the alveolar wall surrounding the root, and affects the quality of bone structure resulting in retardation of its skeletal development.
本研究的目的是评估1型糖尿病（DM）对下颌骨结构以及牙槽/颌骨形成变化的影响。通过单次注射60 mg/kg体重的链脲佐菌素，在3周龄雄性Wistar大鼠中诱导实验性DM。所有大鼠在第21天和第28天注射钙黄绿素。大鼠在8周龄时处死。通过骨组织形态计量学、显微计算机断层扫描（micro-CT）和组织学切片分析骨结构。组织形态计量学分析表明，与对照组相比，DM组大多数下颌区域的矿物质沉积和骨形成率显著降低。Micro-CT分析显示DM大鼠的骨质明显恶化。为了对骨吸收进行组织计量学测量，对牙槽壁远端面的破骨细胞数量进行计数。DM大鼠的破骨细胞数量明显低于对照组。这些发现表明，未控制的DM会减少下颌骨形成，降低牙根周围牙槽壁的骨转换率，并影响骨结构质量，导致骨骼发育迟缓。
