Nardelli Dean T, Luedtke Joshua O, Munson Erik L, Warner Thomas F, Callister Steven M, Schell Ronald F
Department of Health Sciences, University of Wisconsin-Milwaukee, Milwaukee, WI, USA.
FEMS Immunol Med Microbiol. 2010 Oct;60(1):78-89. doi: 10.1111/j.1574-695X.2010.00721.x.
The immunological events leading to the development of Lyme arthritis in humans are partially understood. Much of this information has been gained by studying the course of infection of naïve or vaccinated mice with Borrelia burgdorferi. However, the Borrelia-vaccination and -infection model has not been described using the organismal parameters commonly used in the widely accepted Borrelia-infection model. This is the first comparison between the Borrelia-infection and the Borrelia-vaccination and -infection models of arthritis. Borrelia-vaccinated and -infected C3H/HeN mice develop acute inflammation comparable to that of nonvaccinated, Borrelia-infected C3H/HeN mice. The duration and severity of arthritis in Borrelia-vaccinated and -infected mice was slightly increased compared with Borrelia-infected mice. Significantly, Borrelia-vaccinated and -infected C3H/HeN mice produce interleukin-17 (IL-17), while Borrelia-infected mice that had not been previously vaccinated do not. Neutralization of IL-17 in Borrelia-vaccinated and -infected C3H/HeN mice decreased the severity of arthritis, although not to the degree we observed previously in C57BL/6 mice. Collectively, these findings show that the Borrelia-vaccination and -infection model of Lyme arthritis incorporates elements of adaptive immunity that likely have relevance to human disease, but may not be observed in Borrelia-infected C3H/HeN mice.
导致人类莱姆关节炎发生的免疫事件已部分为人所知。这些信息大多是通过研究未接触过病原体或接种过疫苗的小鼠感染伯氏疏螺旋体的过程获得的。然而，尚未使用广泛接受的伯氏疏螺旋体感染模型中常用的机体参数来描述伯氏疏螺旋体疫苗接种和感染模型。这是首次对伯氏疏螺旋体感染模型与伯氏疏螺旋体疫苗接种及感染性关节炎模型进行比较。接种过伯氏疏螺旋体疫苗并感染的C3H/HeN小鼠会出现与未接种疫苗、感染伯氏疏螺旋体的C3H/HeN小鼠相当的急性炎症。与感染伯氏疏螺旋体的小鼠相比，接种过疫苗并感染的小鼠关节炎的持续时间和严重程度略有增加。值得注意的是，接种过伯氏疏螺旋体疫苗并感染的C3H/HeN小鼠会产生白细胞介素-17（IL-17），而之前未接种过疫苗的感染伯氏疏螺旋体的小鼠则不会。在接种过伯氏疏螺旋体疫苗并感染的C3H/HeN小鼠中中和IL-17可降低关节炎的严重程度，尽管降低程度不如我们之前在C57BL/6小鼠中观察到的那样。总体而言，这些发现表明，莱姆关节炎的伯氏疏螺旋体疫苗接种及感染模型纳入了可能与人类疾病相关的适应性免疫元素，但在感染伯氏疏螺旋体 的C3H/HeN小鼠中可能无法观察到。
