Pan Ai-jun, Jin Kui, Zhou Shu-sheng, Li Chu, Shen Jian-jun, Liu Bao
Intensive Care Unit, Anhui Provincial Hospital Affiliated to Anhui Medical University, Hefei 230001, Anhui, China.
Zhongguo Wei Zhong Bing Ji Jiu Yi Xue. 2010 Jul;22(7):422-5.
To explore the effect of hyperbaric oxygen (HBO) on acute lung injury (ALI) induced by oleic acid (OA) in rats.
Eighty healthy Sprague-Dawley (SD) rats were randomly divided into four groups. In OA group (n=30), ALI was produced by injection of OA 0.15 ml/kg through tail vein. Ten rats were randomly selected and sacrificed after injection of OA at the time of 4 hours, 3 days, and 7 days, respectively. In OA plus HBO group (n=20), rats received HBO intervention in a special box with oxygen of 2.5 atm (1 atm=101.325 kPa) for 90 minutes. Ten rats were randomly respectively sacrificed at 3 days and 7 days. In simple HBO group, 20 rats were sacrificed at 3 days and 7 days of HBO intervention, respectively. Other 10 rats were assigned as control group. Blood, lung specimens were collected after sacrifice. Serum contents of tumor necrosis factor-alpha (TNF-alpha), interleukin-1 beta (IL-1 beta) and IL-6 were measured. Gross changes and pathological findings of the left lung were recorded. The wet to the dry weigh (W/D) of the right lung was determined.
Partial pressure of oxygen in arterial blood (PaO2, mm Hg, 1 mm Hg=0.133 kPa) fell from 107.70+/-5.37 to 57.40+/-2.63 in OA group. Congestion, bleeding and edema could be seen grossly. They could also be found under microscope with disappearance of normal structure, and accumulation of fluid in interstitium with inflammatory cell infiltration and hyaline membrane formation were also found. Lung W/D ratio was increased as compared with the control group (6.94+/-0.44 vs. 4.59+/-0.44, P<0.05). A marked increase was found in serum TNF-alpha, IL-1 beta, IL-6 levels [TNF-alpha (microg/L): 18.52+/-1.20 vs. 5.27+/-0.61, IL-1 beta (microg/L): 13.73+/-1.37 vs. 6.13+/-1.51, IL-6 (microg/L): 14.51+/-1.21 vs. 11.14+/-0.89]. After HBO therapy for 3 days and 7 days, PaO2 (mm Hg, 3 days: 79.20+/-1.68 vs. 59.00+/-2.70, 7 days: 94.30+/-3.77 vs. 74.00+/-3.85) and lung W/D (3 day: 7.43+/-0.73 vs. 9.82+/-0.99, 7 days : 6.75+/-1.14 vs. 8.77+/-1.60) of HBO group were ameliorated to varying degrees compared with OA model group (P<0.05 or P<0.01). HBO therapy for 3 days could lower levels of IL-1 beta (microg/L) in the serum (6.46+/-1.99 vs. 9.09+/-1.09, P<0.05).
It is suggested that HBO treatment for ALI in rats had effects of improving arterial blood gases and the lung water transport, and inhibiting inflammatory mediators production.
探讨高压氧（HBO）对油酸（OA）诱导的大鼠急性肺损伤（ALI）的影响。
将80只健康的Sprague-Dawley（SD）大鼠随机分为四组。OA组（n = 30），通过尾静脉注射0.15 ml/kg OA制备ALI。分别在注射OA后4小时、3天和7天随机选取10只大鼠处死。OA加HBO组（n = 20），大鼠在一个特殊的氧舱内接受2.5个大气压（1个大气压=101.325 kPa）的HBO干预90分钟。分别在3天和7天随机选取10只大鼠处死。单纯HBO组，在HBO干预3天和7天时分别处死20只大鼠。另外10只大鼠作为对照组。处死大鼠后采集血液、肺组织标本。检测血清肿瘤坏死因子-α（TNF-α）、白细胞介素-1β（IL-1β）和IL-6含量。记录左肺大体变化和病理结果。测定右肺湿重与干重之比（W/D）。
OA组动脉血氧分压（PaO2，mmHg，1 mmHg = 0.133 kPa）从107.70±5.37降至57.40±2.63。大体可见充血、出血和水肿。镜下可见正常结构消失，间质积液，有炎性细胞浸润和透明膜形成。与对照组相比，肺W/D比值升高（6.94±0.44 vs. 4.59±0.44，P<0.05）。血清TNF-α、IL-1β、IL-6水平显著升高[TNF-α（μg/L）：18.52±1.20 vs. 5.27±0.61，IL-1β（μg/L）：13.73±1.37 vs. 6.13±1.51，IL-6（μg/L）：14.51±1.21 vs. 11.14±0.89]。HBO治疗3天和7天后，与OA模型组相比，HBO组的PaO2（mmHg，3天：79.20±1.68 vs. 59.00±2.70，7天：94.30±3.77 vs. 74.00±3.85）和肺W/D（3天：7.43±0.73 vs. 9.82±0.99，7天：6.75±1.14 vs. 8.77±1.60）均有不同程度改善（P<0.05或P<0.01）。HBO治疗3天可降低血清中IL-1β（μg/L）水平（6.46±1.99 vs. 9.09±1.09，P<0.05）。
提示HBO治疗大鼠ALI具有改善动脉血气和肺水转运、抑制炎性介质产生的作用。
