一种纯化甲型流感病毒神经氨酸酶的新方法。
A new method for the purification of the influenza A virus neuraminidase.
作者信息
McKimm-Breschkin J L, Caldwell J B, Guthrie R E, Kortt A A
机构信息
CSIRO Division of Biomolecular Engineering, Parkville, Australia.
出版信息
J Virol Methods. 1991 Apr;32(1):121-4. doi: 10.1016/0166-0934(91)90192-3.
A rapid new method for the purification of neuraminidase (NA) heads from influenza A virus is described. Virus was pelleted directly from allantoic fluid and was digested with pronase. The cores were removed by centrifugation, redigested and the released NA heads were pooled and concentrated. The NA was separated from all contaminating proteins in a single step on a Superose 12 column. The purified material was suitable for both crystallography and for the production of monospecific antisera.
描述了一种从甲型流感病毒中纯化神经氨酸酶（NA）头部的快速新方法。病毒直接从尿囊液中沉淀出来，并用链霉蛋白酶消化。通过离心去除核心，再次消化，然后将释放的NA头部合并并浓缩。在Superose 12柱上一步将NA与所有污染蛋白分离。纯化后的物质适用于晶体学研究和单特异性抗血清的制备。
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