具有四臂结构域柄的甲型流感病毒N9神经氨酸酶的结构
Structure of an Influenza A virus N9 neuraminidase with a tetrabrachion-domain stalk.
作者信息
Streltsov Victor A, Schmidt Peter M, McKimm-Breschkin Jennifer L
机构信息
CSIRO Manufacturing, 343 Royal Parade, Parkville, Victoria 3052, Australia.
出版信息
Acta Crystallogr F Struct Biol Commun. 2019 Feb 1;75(Pt 2):89-97. doi: 10.1107/S2053230X18017892. Epub 2019 Jan 23.
Abstract
The influenza neuraminidase (NA) is a homotetramer with head, stalk, transmembrane and cytoplasmic regions. The structure of the NA head with a stalk has never been determined. The NA head from an N9 subtype influenza A virus, A/tern/Australia/G70C/1975 (H1N9), was expressed with an artificial stalk derived from the tetrabrachion (TB) tetramerization domain from Staphylothermus marinus. The NA was successfully crystallized both with and without the TB stalk, and the structures were determined to 2.6 and 2.3 Å resolution, respectively. Comparisons of the two NAs with the native N9 NA structure from egg-grown virus showed that the artificial TB stalk maintained the native NA head structure, supporting previous biological observations.
摘要
流感神经氨酸酶（NA）是一种具有头部、柄部、跨膜区和胞质区的同四聚体。带有柄部的NA头部结构尚未确定。来自甲型N9亚型流感病毒A/tern/澳大利亚/G70C/1975（H1N9）的NA头部与源自海栖热袍菌四臂体（TB）四聚化结构域的人工柄部一起表达。NA在有和没有TB柄部的情况下均成功结晶，其结构分辨率分别为2.6 Å和2.3 Å。将这两种NA与来自鸡胚培养病毒的天然N9 NA结构进行比较，结果表明人工TB柄部保持了天然NA头部结构，这支持了先前的生物学观察结果。
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