Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, CEP 31.270-901 Belo Horizonte, MG, Brazil.
Oncol Rep. 2010 Sep;24(3):677-85. doi: 10.3892/or_00000907.
Breast cancer is a major health burden, responsible for >10% of all cases of cancer worldwide. Advances in breast cancer diagnosis and treatment have contributed to an improved rate of survival, although mortality rates remains significantly high. The establishment of breast cancer cell lines is an important model for understanding biological processes involved in this disease and for identifying potential therapeutic targets. The novel human breast cancer cell lines, MACL-1 and MGSO-3, were used in this study to identify possible surface antigens by antibodies directed against two commercial breast cancer cell lines MCF-7 and MDA-MB-231. We purified a 37 kDa antigen by affinity chromatography from MDA-MB-231, and its N-terminal amino acid sequence was homologous to glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Therefore, immunohistochemical experiments, using specific monoclonal antibodies, evidenced a co-localization of GAPDH and Na+/K+-ATPase on the surface of commercially available and recently established breast cancer cell lines. It is of note that Na+/K+-ATPase was used as a plasma membrane marker. This finding opens new perspectives for breast cancer diagnosis and treatment since GAPDH could be used as a biomarker or as a potential therapeutic target in breast cancer.
乳腺癌是一个重大的健康负担，占全球所有癌症病例的 10%以上。乳腺癌诊断和治疗的进展提高了生存率，尽管死亡率仍然很高。建立乳腺癌细胞系是了解该疾病涉及的生物学过程和确定潜在治疗靶点的重要模型。本研究使用新型人乳腺癌细胞系 MACL-1 和 MGSO-3，通过针对两种商业乳腺癌细胞系 MCF-7 和 MDA-MB-231 的抗体来鉴定可能的表面抗原。我们从 MDA-MB-231 中通过亲和层析纯化了一个 37 kDa 的抗原，其 N 端氨基酸序列与甘油醛-3-磷酸脱氢酶 (GAPDH) 同源。因此，使用特异性单克隆抗体的免疫组织化学实验表明，在商业上可获得的和最近建立的乳腺癌细胞系的表面上，GAPDH 和 Na+/K+-ATPase 共定位。值得注意的是，Na+/K+-ATPase 被用作质膜标记物。这一发现为乳腺癌的诊断和治疗开辟了新的前景，因为 GAPDH 可以用作乳腺癌的生物标志物或潜在的治疗靶点。
