Laboratory of Molecular Neurobiology, Centre for Psychiatric Research, Aarhus University Hospital, Denmark.
Neuroscience. 2010 Oct 27;170(3):731-41. doi: 10.1016/j.neuroscience.2010.07.016. Epub 2010 Jul 24.
Several findings suggest a functional and anatomical differentiation along the dorso-ventral axis of the hippocampus. Lesion studies in rats have indicated that the dorsal hippocampus preferentially plays a role in spatial learning and memory, while the ventral hippocampus is involved in anxiety-related behaviors. Based on such findings our aim was to investigate the molecular differentiation along the dorso-ventral axis of the hippocampal granular cell layer of the rat dentate gyrus. Homogeneous isolation of this specific area was performed by laser-capture microdissection and Illumina microarray chips were used to identify genes differentially expressed in dorsal and ventral granular cell layer, respectively. Selected genes were confirmed by quantitative polymerase chain reaction analyses. From the total amount of 22518 probes 229 genes were found to be differentially expressed between dorsal and ventral granular cell layer with a false discovery rate below 5% and with a relative change in gene expression level of 20% or more. From this pool of genes 45 genes were more than two-fold regulated, 13 genes being dorsally enriched and 32 genes being ventrally enriched. Moreover, cluster analysis based on all genes represented on the microarray chip showed a clear differentiation between dorsal and ventral subgroups. Our findings demonstrate a dorso-ventral differentiation in gene expression even at the subregional level of the rat hippocampus, more specifically in the granular cell layer, substantiating the existence of functional heterogeneity along the dorso-ventral axis of the hippocampus.
有几项研究结果表明，海马体在背腹轴向上存在功能和解剖学的分化。对大鼠的损伤研究表明，背侧海马体优先参与空间学习和记忆，而腹侧海马体则与焦虑相关行为有关。基于这些发现，我们的目的是研究大鼠齿状回颗粒细胞层背腹轴向上的分子分化。通过激光捕获显微切割实现该特定区域的均匀分离，并使用 Illumina 微阵列芯片来分别鉴定背侧和腹侧颗粒细胞层中差异表达的基因。通过定量聚合酶链反应分析来验证选定的基因。在总共 22518 个探针中，有 229 个基因在背侧和腹侧颗粒细胞层之间存在差异表达，假发现率低于 5%，基因表达水平的相对变化为 20%或更高。在这组基因中，有 45 个基因的调节倍数超过两倍，其中 13 个基因在背侧富集，32 个基因在腹侧富集。此外，基于微阵列芯片上所有基因的聚类分析显示，背侧和腹侧亚组之间存在明显的分化。我们的研究结果表明，即使在大鼠海马体的亚区域水平上，也存在基因表达的背腹分化，更具体地说，在颗粒细胞层中存在功能异质性沿海马体的背腹轴。
