College of Food Science and Engineering, Harbin Institute of Technology, Harbin, PR China.
Mol Nutr Food Res. 2011 Feb;55(2):220-8. doi: 10.1002/mnfr.200900593.
Milk basic proteins and bovine colostrum extracts have preventive effects on osteoporosis. However, the effects of bovine colostrum acidic proteins (BCAP) on properties of bone have not been reported. This study investigated the effect of BCAP on the prevention of bone loss in ovariectomized (OVX) rats.
Forty-eight 3-month old female Sprague-Dawley rats were OVX and another 12 rats underwent a sham operation (Sham). The OVX rats were randomly separated into four groups, i.e. OVX control, OVX plus 2 mg/day BCAP, OVX plus 10 mg/day BCAP, and OVX plus 50 mg/day BCAP, and were gavaged once per day for 12 wk. The effects on bone mineral content, bone mineral density, microarchitecture and biomechanical properties were determined. The bioactive ingredients in BCAP were isolated and identified. Results showed that BCAP increased the bone mineral content and bone mineral density of the femur in a dose-dependent manner. Scanning electron microscope observation and mechanical testing further confirmed the positive effects of BCAP. These positive effects attribute to the fact that osteopontin, lactoferrin, epidermal growth factor and insulin-like growth factor-2 are the dominant proteins in BCAP.
BCAP (2-50 mg/day) could prevent osteoporosis caused by bone loss in OVX rats.
牛奶碱性蛋白和牛初乳提取物对骨质疏松症有预防作用。然而，牛初乳酸性蛋白（BCAP）对骨骼性质的影响尚未报道。本研究探讨了 BCAP 对去卵巢（OVX）大鼠骨丢失预防作用。
48 只 3 月龄雌性 Sprague-Dawley 大鼠行卵巢切除术（OVX），另 12 只大鼠行假手术（Sham）。将 OVX 大鼠随机分为 4 组，即 OVX 对照组、OVX 加 2mg/天 BCAP 组、OVX 加 10mg/天 BCAP 组和 OVX 加 50mg/天 BCAP 组，每天灌胃 1 次，共 12 周。测定骨矿物质含量、骨矿物质密度、微结构和生物力学性能的影响。分离并鉴定 BCAP 中的生物活性成分。结果表明，BCAP 呈剂量依赖性增加股骨的骨矿物质含量和骨矿物质密度。扫描电子显微镜观察和力学测试进一步证实了 BCAP 的积极作用。这些积极作用归因于骨桥蛋白、乳铁蛋白、表皮生长因子和胰岛素样生长因子-2 是 BCAP 中的主要蛋白。
BCAP（2-50mg/天）可预防 OVX 大鼠因骨丢失引起的骨质疏松症。
