巴戟天胶囊对去卵巢大鼠骨质疏松症的影响。
Effect of Morinda officinalis capsule on osteoporosis in ovariectomized rats.
作者信息
Li Ye, Lü Shan-Shan, Tang Gui-Ying, Hou Min, Tang Qing, Zhang Xiao-Na, Chen Wei-Hai, Chen Gang, Xue Qiang, Zhang Cong-Cong, Zhang Ji-Fen, Chen Yi, Xu Xiao-Yu
机构信息
College of Pharmaceutical Sciences, Institute of Chinese Medicine, Pharmacology of Chinese Materia Medica-the key constructing discipline by the State Administrative Bureau of TCM, Southwest University, Chongqing 400715, China.
Healn Pharmaceutical Co. Ltd., Chongqing 401520, China.
出版信息
Chin J Nat Med. 2014 Mar;12(3):204-12. doi: 10.1016/S1875-5364(14)60034-0.
AIM
To explore the therapeutic effects of Morinda officinalis capsules (MOP) on osteoporosis in ovariectomized rats.
METHODS
Six-month-old female Sprague-Dawley rats were induced for postmenopausal osteoporosis (PMOP) by bilateral ovariectomy and divided into seven groups as follows: sham-operated group, ovariectomized (OVX) control group, OVX treated with xianlinggubao (XLGB) (270 mg·kg⁻¹·d⁻¹), OVX treated with alendronate sodium (ALN) (3 mg·kg⁻¹·d⁻¹), and OVX treated with Morinda officinalis capsule (MOP) of graded doses (90, 270 and 810 mg·kg⁻¹·d⁻¹) groups. Oral treatments were administered daily on the 4(th) week after ovariectomy and lasted for 12 weeks. The bone mineral density was evaluated by dual-energy X-ray absorptiometry. The tartrate-resistant acid phosphatase (TRAP), alkaline phosphatase (AKP), and osteocalcin (OC) levels in the serum and plasma were determined by standard colorimetric and enzyme immunoassays methods. Bone biomechanical properties and morphological parameters were analyzed by three-point bending test and histomorphometry respectively.
RESULTS
Morinda officinalis capsules at all doses were able to significantly prevent the OVX-induced loss of bone mass due to diminishing serum AKP and TRAP levels while elevating OC level in the plasma. Morinda officinalis capsules also enhanced the bone strength and prevented the deterioration of trabecular microarchitecture.
CONCLUSION
Morinda officinalis capsules possess potent anti-osteoporotic activity in OVX rats which could be an effective treatment for postmenopausal osteoporosis.
目的
探讨巴戟天胶囊对去卵巢大鼠骨质疏松症的治疗作用。
方法
6月龄雌性Sprague-Dawley大鼠通过双侧卵巢切除术诱导绝经后骨质疏松症（PMOP），并分为以下七组：假手术组、去卵巢（OVX）对照组、用仙灵骨葆（XLGB）（270 mg·kg⁻¹·d⁻¹）治疗的OVX组、用阿仑膦酸钠（ALN）（3 mg·kg⁻¹·d⁻¹）治疗的OVX组以及用不同剂量（90、270和810 mg·kg⁻¹·d⁻¹）巴戟天胶囊治疗的OVX组。在卵巢切除术后第4周开始每日进行口服给药，持续12周。通过双能X线吸收法评估骨密度。采用标准比色法和酶免疫分析法测定血清和血浆中抗酒石酸酸性磷酸酶（TRAP）、碱性磷酸酶（AKP）和骨钙素（OC）水平。分别通过三点弯曲试验和组织形态计量学分析骨生物力学性能和形态学参数。
结果
所有剂量的巴戟天胶囊均能通过降低血清AKP和TRAP水平，同时提高血浆OC水平，显著预防OVX诱导的骨量丢失。巴戟天胶囊还增强了骨强度并防止小梁微结构恶化。
结论
巴戟天胶囊对去卵巢大鼠具有显著的抗骨质疏松活性，可能是绝经后骨质疏松症的有效治疗方法。
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