The Division of Molecular and Clinical Gerontology, The Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, Beppu, Oita, 874-0838, Japan.
Aging Male. 2011 Mar;14(1):21-6. doi: 10.3109/13685538.2010.502270. Epub 2010 Jul 29.
The telomere length and subtelomeric methylated status of peripheral blood leukocytes has been reported to be correlated with many kinds of pathophysiological conditions. However, the correlation between the telomeric parameters and clinical laboratory data in metabolic disorders is not well known. This study investigated the correlation between the telomere length and subtelomeric methylated status in peripheral leukocytes and the laboratory data of male outpatients with combined metabolic disorders and no clinical history of cardiovascular or cerebrovascular event were assessed, to find good clinical laboratory markers reflecting the biological aging. The laboratory data were collected in 26 Japanese male outpatients with diabetes mellitus and hyperlipidemia, and no history of cardiovascular or cerebrovascular event, and the telomeric parameters in their peripheral leukocytes were determined by Southern blot with methylation-sensitive and insensitive isoschizomers. Any correlations between the laboratory data and the telomeric parameters were assessed. The patients showed a significant negative correlation among the bilirubin and creatine phosphokinase with the aging-associate change of the telomeric and subtelomeric parameters. Lowered serum bilirubin and creatinine phosphokinase level correlated to genomic aging represented by telomere attrition of patients with metabolic disorders.
外周血白细胞端粒长度和亚端粒甲基化状态与许多病理生理状况相关已经被报道。然而，端粒参数与代谢紊乱患者的临床实验室数据之间的相关性尚不清楚。本研究调查了外周血白细胞中端粒长度和亚端粒甲基化状态与患有合并代谢紊乱且无心血管或脑血管事件临床病史的男性门诊患者的实验室数据之间的相关性，以寻找反映生物学年龄的良好的临床实验室标志物。在 26 名患有糖尿病和高脂血症但无心血管或脑血管事件病史的日本男性门诊患者中收集了实验室数据，并通过Southern blot 用甲基化敏感和不敏感的同裂酶来测定外周白细胞中端粒参数。评估了实验室数据与端粒参数之间的任何相关性。患者的胆红素和肌酸磷酸激酶之间存在显著的负相关，与端粒和亚端粒参数的与衰老相关的变化相关。代谢紊乱患者血清胆红素和肌酸磷酸激酶水平降低与端粒损耗相关，代表基因组衰老。
