Division of Molecular and Clinical Gerontology, Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, Oita, Japan.
J Nutr Health Aging. 2010 Nov;14(9):793-7. doi: 10.1007/s12603-010-0136-4.
To elucidate the correlation between the telomere length and subtelomeric methylated status in peripheral leukocytes and the laboratory data of inpatients with brain infarction and metabolic disorders. This is the first report describing a link between routine clinical laboratory data and genomic aging.
Cross-sectional population-based study.
Chronic disease ward of Kyushu University Hospital at Beppu in Japan.
Inpatients with brain infarction and metabolic disorders.
The laboratory data of male patients were collected and the telomeric parameters in their peripheral leukocytes were determined by a Southern blot analysis with methylation-sensitive and insensitive isoschizomers. Any correlations between the laboratory data and the telomeric parameters were assessed.
The patients revealed a significant correlation among the fasting blood sugar, HbA1c, serum creatinine and urea nitrogen levels with the mean telomere length, expression of long telomeres ( > 9.4 kb), or the subtelomeric hypermethylation status of long telomeres.
Our results suggested that the hyperglycemia and renal function of patients with metabolic disorders correlated positively with the aging-associated telomeric changes.
阐明外周血白细胞端粒长度与亚端粒甲基化状态与脑梗死和代谢紊乱住院患者的实验室数据之间的相关性。这是首次描述常规临床实验室数据与基因组衰老之间联系的报告。
基于横断面的人群研究。
日本别府九州大学医院慢性病病房。
脑梗死和代谢紊乱的住院患者。
收集男性患者的实验室数据，并通过使用甲基化敏感和不敏感同裂酶的Southern 印迹分析来确定其外周血白细胞中的端粒参数。评估实验室数据与端粒参数之间的任何相关性。
患者的空腹血糖、HbA1c、血清肌酐和尿素氮水平与端粒长度平均值、长端粒（>9.4kb）的表达或长端粒的亚端粒高甲基化状态之间存在显著相关性。
我们的结果表明，代谢紊乱患者的高血糖和肾功能与衰老相关的端粒变化呈正相关。
