Bioconversion of silver salt into silver nanoparticles using different microorganisms.

Abstract: Recently, silver nanoparticles have generated enough interest due to their immense usage. Until now chemical synthesis has been a fast method to produce nanoparticles, but the release of environmental pollutants has raised caution. So a more subtle biochemical approach is in research. Three microorganisms, Aspergillus flavus, Phoma exigua and Bacillus megaterium, were selected to reduce silver nitrate to silver nanoparticles in aqueous form. All three microorganisms showed varying reducing capacity, of which Phoma exigua showed the highest, when their biomass was incubated in silver nitrate for 48 hours. The nanoparticles showed all the optical and physical properties, which were analyzed by measuring the surface plasmon resonance and TEM. It was also observed that the fungal species have varied growth in silver nitrate and can be used to produce silver nanoparticles, directly incubating the inocula of fungus in the media containing silver nitrate. On comparing all the characteristics and results produced, Aspergillus flavus was found to be the most effective microorganism, which can convert the silver nitrate to silver nanoparticles in aqueous condition. The bacterial species showed no growth with incubation with silver nitrate.