Section of Molecular Cell and Developmental Biology, Institute for Cellular and Molecular Biology, University of Texas, Austin, Texas 78712, USA.
Plant Physiol. 2010 Oct;154(2):949-57. doi: 10.1104/pp.110.161083. Epub 2010 Jul 29.
VERNALIZATION INSENSITIVE3 (VIN3) induction by vernalization is one of the earliest events in the vernalization response of Arabidopsis (Arabidopsis thaliana). However, the mechanism responsible for vernalization-mediated VIN3 induction is poorly understood. Here, we show that the constitutive repression of VIN3 in the absence of the cold is due to multiple repressive components, including a transposable element-derived sequence, LIKE-HETEROCHROMATIN PROTEIN1 and POLYCOMB REPRESSION COMPLEX2. Furthermore, the full extent of VIN3 induction by vernalization requires activating complex components, including EARLY FLOWERING7 and EARLY FLOWERING IN SHORT DAYS. In addition, we observed dynamic changes in the histone modifications present at VIN3 chromatin during the course of vernalization. Our results show that the induction of VIN3 includes dynamic changes at the level of chromatin triggered by long-term cold exposure.
春化素不敏感 3 型（VIN3）的诱导是拟南芥（Arabidopsis thaliana）春化反应中最早的事件之一。然而，春化介导的 VIN3 诱导的机制尚不清楚。在这里，我们表明，在没有寒冷的情况下，VIN3 的组成性抑制是由于多个抑制成分，包括转座元件衍生序列、类似异染色质蛋白 1 和多梳抑制复合物 2。此外，春化诱导 VIN3 的完全程度需要激活复合物成分，包括早期开花 7 和短日照下的早期开花。此外，我们在春化过程中观察到 VIN3 染色质上存在的组蛋白修饰的动态变化。我们的结果表明，VIN3 的诱导包括长期低温暴露触发的染色质水平的动态变化。
