剪接体中翻译后修饰的鸟瞰图及其在剪接体动力学中的作用。
A bird's-eye view of post-translational modifications in the spliceosome and their roles in spliceosome dynamics.
作者信息
McKay Susannah L, Johnson Tracy L
机构信息
Division of Biological Sciences, Molecular Biology Section MC-0377, 9500 Gilman Drive, La Jolla, CA 92093-0377, USA.
出版信息
Mol Biosyst. 2010 Nov;6(11):2093-102. doi: 10.1039/c002828b. Epub 2010 Jul 29.
Abstract
Pre-mRNA splicing, the removal of noncoding intron sequences from the pre-mRNA, is a critical reaction in eukaryotic gene expression. Pre-mRNA splicing is carried out by a remarkable macromolecular machine, the spliceosome, which undergoes dynamic rearrangements of its RNA and protein components to assemble its catalytic center. While significant progress has been made in describing the "moving parts" of this machine, the mechanisms by which spliceosomal proteins mediate the ordered rearrangements within the spliceosome remain elusive. Here we explore recent evidence from proteomics studies revealing extensive post-translational modification of splicing factors. While the functional significance of most of these modifications remains to be characterized, we describe recent studies in which the roles of specific post-translational modifications of splicing factors have been characterized. These examples illustrate the importance of post-translational modifications in spliceosome dynamics.
摘要
前体信使核糖核酸（pre-mRNA）剪接，即从前体mRNA中去除非编码内含子序列，是真核基因表达中的关键反应。前体mRNA剪接由一种非凡的大分子机器——剪接体完成，剪接体的RNA和蛋白质成分会发生动态重排以组装其催化中心。虽然在描述这一机器的“活动部件”方面已取得显著进展，但剪接体蛋白介导剪接体内有序重排的机制仍不清楚。在此，我们探讨蛋白质组学研究的最新证据，这些证据揭示了剪接因子广泛的翻译后修饰。虽然这些修饰大多的功能意义仍有待确定，但我们描述了最近一些对剪接因子特定翻译后修饰作用进行了表征的研究。这些例子说明了翻译后修饰在剪接体动力学中的重要性。
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