Chinnadurai G, Chinnadurai S, Green M
J Virol. 1978 Apr;26(1):195-9. doi: 10.1128/JVI.26.1.195-199.1978.
The infectivity of adenovirus type 2 DNA and a DNA-protein complex was studied in 293 cells, a human embryonic kidney cell line transformed by sheared adenovirus type 5 DNA, and in human KB cells. Adenovirus type 2 DNA was more infectious (up to about 40-fold) in 293 cells than in KB cells, whereas a DNA-protein complex (prepared by a rapid procedure) had about the same infectivity in both cell lines. These data may mean that a factor present in 293 cells (perhaps a viral-coded protein) enhances the infectivity of free viral DNA. The infectivity of DNA and the DNA-protein complex was increased up to fivefold by brief treatment of cell monolayers with 25% dimethyl sulfoxide after transfection. Under these conditions, (i) the infectivity of native adenovirus type 2 DNA ranged from 400 to 1,300 PFU/microgram of DNA in 293 cells and from about 9 to 14 PFU/microgram of DNA in KB cells, and (ii) the infectivity of the DNA-protein complex was 6 X 10(3)to 2 X 10(4) PFU/microgram in 293 cells and 1.4 X 10(4) to 1.6 X 10(4) PFU/microgram in KB cells.
在293细胞（一种由剪切的腺病毒5型DNA转化的人胚肾细胞系）和人KB细胞中研究了腺病毒2型DNA及一种DNA - 蛋白质复合物的感染性。腺病毒2型DNA在293细胞中的感染性比在KB细胞中高（高达约40倍），而一种（通过快速方法制备的）DNA - 蛋白质复合物在两种细胞系中的感染性大致相同。这些数据可能意味着293细胞中存在的一种因子（可能是一种病毒编码蛋白）增强了游离病毒DNA的感染性。转染后用25%二甲基亚砜短暂处理细胞单层，DNA及DNA - 蛋白质复合物的感染性增加了五倍。在这些条件下，（i）天然腺病毒2型DNA在293细胞中的感染性为每微克DNA 400至1300个空斑形成单位（PFU），在KB细胞中为每微克DNA约9至14个PFU；（ii）DNA - 蛋白质复合物在293细胞中的感染性为每微克6×10³至2×10⁴个PFU，在KB细胞中为每微克1.4×10⁴至1.6×10⁴个PFU。
