Mirza A, Weber J
Intervirology. 1980;13(5):307-11. doi: 10.1159/000149139.
A temperature-sensitive mutant of adenovirus type 2 grown at 39 degrees (tsl-39 degrees) produces noninfectious physical particles. DNA and core structures derived from such virion particles, however, were found to be infectious at the permissive temperature. Calcium chloride mediated transfection showed that core structures were 20-40 times more infectious than deprotenized DNA. Because in vivo the noninfectious ts1-39 degrees virions are blocked in uncoating at the core-like stage, we attempted to determine the reason for the infectivity of tsl-39 degrees cores prepared in vitro by investigating the metabolic fate of labeled cores in transfected cells. As opposed to infection with virus, the tsl-39 degrees cores were found to penetrate the nucleus efficiently and uncoat by shedding their core proteins. A possible explanation for the infectivity of the cores is discussed.
在39℃培养的2型腺病毒温度敏感突变体（ts1-39℃）产生无感染性的物理颗粒。然而，发现源自此类病毒体颗粒的DNA和核心结构在允许温度下具有感染性。氯化钙介导的转染表明，核心结构的感染性比脱蛋白DNA高20至40倍。由于在体内，无感染性的ts1-39℃病毒体在类核心阶段的脱壳过程中受阻，我们试图通过研究转染细胞中标记核心的代谢命运，来确定体外制备的ts1-39℃核心具有感染性的原因。与病毒感染不同，发现ts1-39℃核心能有效穿透细胞核并通过脱去核心蛋白进行脱壳。文中讨论了核心具有感染性的一种可能解释。
