Siegl G, Frösner G G
J Virol. 1978 Apr;26(1):40-7. doi: 10.1128/JVI.26.1.40-47.1978.
Virus-like particles were purified from stools of patients in an epidemic of hepatitis A in Germany. When reference MS-1 chimpanzee pre-inoculation and convalescent sera were used, the close serological relationship of the purified particles to well-known isolates of hepatitis A could be established. On the other hand, the physicochemical characteristics of the particles were determined in parallel to the characteristics of a marker parvovirus (LuIII) and a marker picornavirus (poliovirus type 2). It could be shown that the majority of the hepatitis A-associated particles band at 1.34 g/ml in CsCl and, like poliovirus, sediment at about 160S. In addition, a distinct hepatitis A antigen was observed, which banded at 1.305 g/ml and sedimented between 50 and 90S. A further component accumulated in the density range of between 1.38 and 1.44 g/ml. However, it seemed to be rather labile. Upon reisolation from CsCl and sedimentation in sucrose, it resolved into a 160S, a 90 to 100S, and a 50S form. The size of the 160S particles (27 to 29 nm) could be readily distinguished from that of the parvovirus (22 to 24 nm). It is concluded, therefore, that hepatitis A-associated virus particles are more likely to be classified with the picornaviruses than with the parvoviruses.
从德国一次甲型肝炎流行期间患者的粪便中纯化出了病毒样颗粒。当使用参考MS - 1黑猩猩接种前和恢复期血清时，可确定纯化颗粒与甲型肝炎知名分离株之间存在密切的血清学关系。另一方面，同时测定了这些颗粒的物理化学特性以及一种标记细小病毒（LuIII）和一种标记微小核糖核酸病毒（脊髓灰质炎病毒2型）的特性。结果表明，大多数与甲型肝炎相关的颗粒在CsCl中的密度为1.34 g/ml，并且像脊髓灰质炎病毒一样，沉降系数约为160S。此外，观察到一种独特的甲型肝炎抗原，其密度为1.305 g/ml，沉降系数在50至90S之间。另一种成分在密度范围为1.38至1.44 g/ml之间积累。然而，它似乎相当不稳定。从CsCl中重新分离并在蔗糖中沉降后，它分解为160S、90至100S和50S三种形式。160S颗粒的大小（27至29 nm）可以很容易地与细小病毒的大小（22至24 nm）区分开来。因此得出结论，与甲型肝炎相关的病毒颗粒更有可能归类于微小核糖核酸病毒而不是细小病毒。
