甲型肝炎病毒在实验感染黑猩猩中的周期性排泄：相关甲型肝炎病毒颗粒的生物物理特性

Cyclic excretion of hepatitis A virus in experimentally infected chimpanzees: biophysical characterization of the associated HAV particles.

作者信息

Bradley D W, Gravelle C R, Cook E H, Fields R M, Maynard J E

出版信息

J Med Virol. 1977;1(2):133-8. doi: 10.1002/jmv.1890010206.

PMID:205631

Abstract

Experimental infection of two chimpanzees with the Phoenix Antigen strain of HAV resulted in the cyclic excretion of virus particles on days 9-11, 14-15, and 20-21 postinoculation. Isopycnic banding in CsCl of stool suspensions prepared from 9-11; 14-15; and 17, 19, 21 dav stool pools revealed multiple buoyant densities for the associated HAV particles. Hollow HAV particles found in the 9-11 day pool banded primarily at a buoyant density of 1.30 g/cm3. HAV in the 14-15 day stool banded bimodally in a CsCl gradient, with antigen peaks at buoyant densities of 1.29 and 1.33 g/cm3. HAV in the days 17, 19, 21 stool pool also banded bimodally in a CsCl gradient; however, the antigen peaks occurred at buoyant densities of 1.33 and 1.40 g/cm3.

摘要

用甲型肝炎病毒（HAV）的凤凰抗原株对两只黑猩猩进行实验性感染，结果在接种后第9 - 11天、14 - 15天和20 - 21天出现病毒颗粒的周期性排泄。对从第9 - 11天、14 - 15天以及第17、19、21天的粪便样本制备的粪便悬液进行氯化铯等密度梯度离心，结果显示相关HAV颗粒具有多种浮力密度。在第9 - 11天的样本池中发现的空心HAV颗粒主要以1.30 g/cm³的浮力密度形成条带。第14 - 15天粪便中的HAV在氯化铯梯度中呈双峰分布，抗原峰出现在1.29和1.33 g/cm³的浮力密度处。第17、19、21天粪便样本池中的HAV在氯化铯梯度中也呈双峰分布；然而，抗原峰出现在1.33和1.40 g/cm³的浮力密度处。