Moghaddas Omid, Seyedjafari Ehsan, Mahoutchi Donya Sadat
Periodontist, Private Practice, Tehran, Iran.
Department of Biotechnology, College of Science, University of Tehran, Tehran, Iran.
J Adv Periodontol Implant Dent. 2024 Aug 11;16(2):133-138. doi: 10.34172/japid.2024.015. eCollection 2024.
Acellular dermal matrix (ADM) has been introduced as an alternative to autogenous grafts. This study assessed the biological behavior of mesenchymal stem cells (MSCs) on two types of commercial ADM scaffolds.
The present in vitro study investigated the behavior of MSCs cultured on scaffold type I CenoDerm® (Tissue Regeneration Corporation) and type II Acellular Dermis (Iranian Tissue Product Co.) as the test groups and an empty well plate as the control group (n=78). Cell attachment was assessed after 12 hours of incubation using 6,4-diamidino-2-phenylindole (DAPI) staining and methyl thiazole tetrazolium (MTT) assay. Cell proliferation was assessed using the MTT assay at 24- and 84-hour and 7-day intervals. Cell morphology was also assessed under a scanning electron microscope (SEM) at 24 hours. MTT assay and DAPI staining were repeated for five samples in all the three groups. Mann-Whitney, ANOVA, and post hoc Tukey tests were used for statistical analysis.
The DAPI staining and MTT assay showed similar results concerning cell attachment between all the groups at 12 hours (=0.4). At 24 hours, cell proliferation was significantly higher in scaffold groups (<0.001). At seven days, the lowest cell proliferation was noted in the scaffold II group, with a significant difference between the groups (=0.01). At 24 hours, cell expansion was greater in the control group, followed by the scaffold I group.
Both scaffolds were similar in MSC attachment, but scaffold I appeared superior to scaffold II in terms of MSC proliferation and morphology in vitro.
脱细胞真皮基质（ADM）已被引入作为自体移植物的替代品。本研究评估了间充质干细胞（MSCs）在两种商用ADM支架上的生物学行为。
本体外研究调查了在I型CenoDerm®（组织再生公司）和II型脱细胞真皮（伊朗组织产品公司）支架上培养的MSCs的行为作为测试组，以空孔板作为对照组（n = 78）。孵育12小时后，使用6,4-二脒基-2-苯基吲哚（DAPI）染色和甲基噻唑四氮唑（MTT）测定法评估细胞附着情况。在24小时、84小时和7天的间隔时间使用MTT测定法评估细胞增殖。在24小时时还通过扫描电子显微镜（SEM）评估细胞形态。对所有三组中的五个样品重复进行MTT测定和DAPI染色。使用Mann-Whitney检验、方差分析和事后Tukey检验进行统计分析。
DAPI染色和MTT测定在12小时时显示所有组之间细胞附着情况的结果相似（P = 0.4）。在24小时时，支架组中的细胞增殖显著更高（P < 0.001）。在七天时，支架II组中观察到最低的细胞增殖，组间存在显著差异（P = 0.01）。在24小时时，对照组中的细胞扩增更大，其次是支架I组。
两种支架在MSCs附着方面相似，但在体外MSCs增殖和形态方面，支架I似乎优于支架II。
