The Vocational High School of Bafra, University of Ondokuz Mayis, Samsun, Turkey.
Pharm Biol. 2010 Aug;48(8):906-14. doi: 10.3109/13880200903311136.
Hypericum perforatum L. (Guttiferae) contains many bioactive secondary metabolites including hypericins, hyperforins, and essential oil.
The present study was conducted to determine the variation in composition of essential oil in H. perforatum accessions from Turkey.
At full flowering, aerial parts of 30 plants were collected from 10 sites of northern Turkey and assayed for essential oil components by GC-FID and GC-MS.
The chemical analysis revealed that the main constituents of the all analyzed samples were hydrocarbon and oxygenated sesquiterpenes such as beta-caryophyllene (4.08-5.93%), gamma-muurolene (5.00-9.56%), beta-selinene (5.08-19.63%), alpha-selinene (4.12-10.42%), d-cadinene (3.02-4.94%), spathulenol (2.34-5.14%), and caryophyllene oxide (6.01-12.18%). Monoterpenes, both hydrocarbon and oxygenated, were represented by scarce amounts of alpha- and beta-pinene, myrcene, linalool, cis- and trans-linalool oxide, and alpha-terpineol. Principal component analysis was also carried out and, according to the results, the first nine principal components were found to represent 100% of the observed variation.
The chemical variation among the populations is discussed as the possible result of different genetic and environmental factors.
The wild populations examined here are potentially important sources for breeding and improvement of the cultivated varieties.
贯叶金丝桃（藤黄科）含有许多生物活性次生代谢产物，包括贯叶金丝桃素、金丝桃素和精油。
本研究旨在确定土耳其贯叶金丝桃品种精油成分的变化。
在盛花期，从土耳其北部的 10 个地点采集了 30 株植物的地上部分，并用 GC-FID 和 GC-MS 测定其精油成分。
化学分析表明，所有分析样品的主要成分是烃类和含氧倍半萜类，如β-石竹烯（4.08-5.93%）、γ-桉叶油烯（5.00-9.56%）、β-榄香烯（5.08-19.63%）、α-榄香烯（4.12-10.42%）、d-杜松烯（3.02-4.94%）、斯巴醇（2.34-5.14%）和石竹烯氧化物（6.01-12.18%）。单萜类化合物，包括烃类和含氧类，只以微量的α-和β-蒎烯、月桂烯、芳樟醇、顺式和反式芳樟醇氧化物以及α-萜品醇存在。还进行了主成分分析，根据结果，发现前九个主成分代表了观察到的变异的 100%。
种群之间的化学变异被认为是不同遗传和环境因素的可能结果。
本文研究的野生种群可能是培育和改良栽培品种的重要资源。
