Başer K Hüsnü Can, Demirci Betül, Demirci Fatih, Hashimoto Toshihoro, Asakawa Yoshinori, Noma Yoshiaki
Planta Med. 2002 Jun;68(6):564-7. doi: 10.1055/s-2002-32557.
Essential oil obtained by micro-distillation and hydrodistillation of the endemic Ferulago thirkeana (Boiss.) Boiss. (Apiaceae) was analysed by GC/MS. An unknown component isolated from the essential oil was characterized by chromato-spectral techniques (1D-, 2D-NMR, HRMS, IR and UV) as (1 S)-2,6,6-trimethyl-4-oxobicyclo[3.1.1]hept-2-enyl(2 E)-2-methylbut-2-enoate (= ferulagone or 1-angeloyloxyverbenone) (1). Biological activities of the essential oil and the purified major component (1) were tested against various human pathogenic microorganisms resulting in moderate inhibition (62.5 - 125 microg/ml).
通过对伞形科特有植物费鲁拉戈草（Ferulago thirkeana (Boiss.) Boiss.）进行微量蒸馏和水蒸馏获得的精油，采用气相色谱/质谱联用（GC/MS）进行分析。从该精油中分离出的一种未知成分，通过色谱光谱技术（一维、二维核磁共振、高分辨质谱、红外光谱和紫外光谱）鉴定为(1S)-2,6,6-三甲基-4-氧代双环[3.1.1]庚-2-烯基(2E)-2-甲基-2-丁烯酸酯（=费鲁拉酮或1-当归酰氧基马鞭草烯酮）（1）。对该精油及其纯化后的主要成分（1）针对多种人类致病微生物的生物活性进行了测试，结果显示有中等程度的抑制作用（62.5 - 125微克/毫升）。
