Theriot J A, Mitchison T J
Department of Biochemistry, University of California, San Francisco 94143-0448.
Nature. 1991 Jul 11;352(6331):126-31. doi: 10.1038/352126a0.
The dynamic behaviour of actin filaments has been directly observed in living, motile cells using fluorescence photoactivation. In goldfish epithelial keratocytes, the actin microfilaments in the lamellipodium remain approximately fixed relative to the substrate as the cell moves over them, regardless of cell speed. The rate of turnover of actin subunits in the lamellipodium is remarkably rapid. Cell movement is directly and tightly coupled to the formation of new actin filaments at the leading edge.
利用荧光光激活技术，已在活的运动细胞中直接观察到肌动蛋白丝的动态行为。在金鱼上皮角膜细胞中，当细胞在其上方移动时，片状伪足中的肌动蛋白微丝相对于底物大致保持固定，而与细胞速度无关。片状伪足中肌动蛋白亚基的周转速度非常快。细胞运动与前缘新肌动蛋白丝的形成直接且紧密相关。
