Rice University, Department of Chemistry, 6100 Main Street, Mail Stop 60, Houston, TX 77005, USA.
Chem Soc Rev. 2010 Sep;39(9):3510-27. doi: 10.1039/b919455j. Epub 2010 Jul 30.
Collagen is a fascinating system of proteins that undergo a multi-step, hierarchical self-assembly which starts from individual peptide chains that assemble into a canonical triple helix. These triple helices then assemble into higher order structures which are often, but not always, fibrous in nature. While collagen is the most abundant protein in the human body, the details of its structure and mechanism of assembly are surprisingly poorly understood. This critical review will focus on small peptide systems, commonly referred to as collagen mimetic peptides (CMPs) which have been used successfully to help unravel some of the mystery of this complex structure. We will discuss homotrimeric CMPs, which are the most commonly researched subject in this field, and the structure of the collagen triple helix in detail and the factors that contribute to its stabilization. We will also cover how CMPs have been used to study breaks in triple helical domains as models for connective tissue diseases and, finally, how they have been used to understand the interactions of collagenous proteins with cell-surface receptors. Additionally, we will focus on heterotrimeric CMPs, a relatively new area of collagen research. Finally, we will deal with CMPs used as models for higher level self-assembly and also as materials that are designed to mimic the function of collagens in the extracellular matrix (178 references).
胶原蛋白是一种令人着迷的蛋白质系统，经历了多步、层次分明的自组装过程，从单个肽链开始，组装成典型的三螺旋。这些三螺旋然后组装成更高阶的结构，这些结构通常但不总是纤维状的。虽然胶原蛋白是人体内最丰富的蛋白质，但它的结构和组装机制的细节却出人意料地知之甚少。这篇重要的综述将集中讨论小肽系统，通常称为胶原蛋白模拟肽（CMP），这些肽已被成功地用于帮助揭开这个复杂结构的一些奥秘。我们将详细讨论同三聚体 CMP，这是该领域最常研究的课题，以及胶原三螺旋的结构及其稳定的因素。我们还将介绍 CMP 如何被用来研究作为结缔组织疾病模型的三螺旋结构域的断裂，以及它们如何被用来理解胶原蛋白与细胞表面受体的相互作用。此外，我们将重点介绍异三聚体 CMP，这是胶原蛋白研究的一个相对较新的领域。最后，我们将讨论用作更高层次自组装模型的 CMP，以及旨在模拟细胞外基质中胶原蛋白功能的材料（178 篇参考文献）。
