Yao Linyan, He Manman, Li Dongfang, Tian Jing, Liu Huanxiang, Xiao Jianxi
State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Meta Chemistry and Resources Utilization of Gansu Province, College of Chemistry and Chemical Engineering, Lanzhou University Lanzhou 730000 P. R. China
School of Pharmacy, Lanzhou University Lanzhou 730000 P. R. China.
RSC Adv. 2018 Jan 10;8(5):2404-2409. doi: 10.1039/c7ra11855d. eCollection 2018 Jan 9.
The development of novel strategies to construct collagen mimetic peptides capable of self-assembling into higher-order structures plays a critical role in the discovery of functional biomaterials. We herein report the construction of a novel type of amphiphile-like peptide conjugating the repetitive triple helical (GPO) sequences characteristic of collagen with terminal hydrophilic aspartic acids. The amphiphile-like collagen mimic peptides containing a variable length of (Gly-Pro-Hyp) sequences consistently generate well-ordered nanospherical supramolecular structures. The C-terminal aspartic acids have been revealed to play a determinant role in the appropriate self-assembly of amphiphile-like collagen mimic peptides. Their presence is a prerequisite for self-assembly, and their lengths could modulate the morphology of final assemblies. We have demonstrated for the first time that amphiphile-like collagen mimic peptides with terminal aspartic acids may provide a general and convenient strategy to create well-defined nanostructures in addition to amphiphile-like peptides utilizing β-sheet or α-helical coiled-coil motifs. The newly developed assembly strategy together with the ubiquitous natural function of collagen may lead to the generation of novel improved biomaterials.
开发能够自组装成高阶结构的新型胶原模拟肽策略，在功能性生物材料的发现中起着关键作用。我们在此报告了一种新型两亲性肽的构建，该肽将胶原特有的重复三螺旋（GPO）序列与末端亲水性天冬氨酸结合。含有可变长度（Gly-Pro-Hyp）序列的两亲性胶原模拟肽始终能形成有序的纳米球形超分子结构。已揭示C末端天冬氨酸在两亲性胶原模拟肽的适当自组装中起决定性作用。它们的存在是自组装的先决条件，其长度可调节最终组装体的形态。我们首次证明，具有末端天冬氨酸的两亲性胶原模拟肽除了利用β-折叠或α-螺旋卷曲螺旋基序的两亲性肽外，还可能提供一种通用且便捷的策略来创建明确的纳米结构。新开发的组装策略与胶原普遍存在的天然功能相结合，可能会产生新型改良生物材料。
