Advanced Magnetic Resonance Center, Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma 73104, USA.
J Magn Reson Imaging. 2010 Aug;32(2):267-75. doi: 10.1002/jmri.22263.
To evaluate the added value of non-contrast-enhanced MR angiography (MRA) to conventional MR imaging for a detailed characterization of different rodent glioma models.
Intracerebral tumor cell implantation and chemical induction methods were implemented to obtain rat C6, 9L/LacZ, F98, RG2, and ethyl-nitrosourea (ENU) -induced glioma models, a human U87 MG tumor model as well as a mouse GL261 glioma model. MR assessments were regularly conducted on a 7 Tesla Bruker BioSpin system. The tumor border sharpness and growth characteristics of each glioma model were assessed from T(2)-weighted images. Neovascularization and vascular alterations inherent to each model were characterized by assessing absolute blood volumes, vessel density, length, and diameter using Mathematica and Amira software.
The 9L/LacZ and ENU gliomas both presented flaws that hinder their use as reliable brain tumor models. C6 gliomas were slightly invasive and induced moderate vascular alterations, whereas GL261 tumors dramatically altered the brain vessels in the glioma region. F98, RG2, and U87 are infiltrative models that produced dramatic vascular alterations.
MRI and MRA provided crucial in vivo information to identify a distinctive "fingerprint" for each of our seven rodent glioma models.
评估非对比增强磁共振血管成像（MRA）对常规磁共振成像的附加价值，以详细描述不同的啮齿动物胶质瘤模型。
采用颅内肿瘤细胞植入和化学诱导方法，获得大鼠 C6、9L/LacZ、F98、RG2 和乙基亚硝脲（ENU）诱导的胶质瘤模型、人 U87 MG 肿瘤模型以及小鼠 GL261 胶质瘤模型。MR 评估定期在 7T Bruker BioSpin 系统上进行。从 T2 加权图像评估每个胶质瘤模型的肿瘤边界清晰度和生长特征。使用 Mathematica 和 Amira 软件评估绝对血容量、血管密度、长度和直径，以表征每个模型的新生血管和固有血管改变。
9L/LacZ 和 ENU 胶质瘤均存在缺陷，使其难以作为可靠的脑肿瘤模型。C6 胶质瘤有轻微侵袭性，引起中度血管改变，而 GL261 肿瘤则明显改变了胶质瘤区域的血管。F98、RG2 和 U87 是浸润性模型，引起了明显的血管改变。
MRI 和 MRA 提供了重要的体内信息，可识别我们的七个啮齿动物胶质瘤模型中的每一个独特的“指纹”。
