Schifter S
Department of Clinical Physiology and Nuclear Medicine, Rigshospitalet, Copenhagen, Denmark.
Peptides. 1991 Mar-Apr;12(2):365-9. doi: 10.1016/0196-9781(91)90027-m.
A highly sensitive and specific radioimmunoassay for human calcitonin gene-related peptide (CGRP) has been developed and used for determination of serum CGRP levels in 232 normal individuals, 123 females and 109 males, range of age 18 to 79 years. Mean serum concentration was 36.3 pmol/l +/- 6.2 (SD), and serum levels were unrelated to age and sex. The specificity of the assay was confirmed by gel chromatography, followed by HPLC analysis of extracts from normal serum using synthetic alpha- and beta-CGRP as standards.
一种用于检测人降钙素基因相关肽（CGRP）的高灵敏度和特异性放射免疫分析法已被开发出来，并用于测定232名正常个体（123名女性和109名男性，年龄在18至79岁之间）的血清CGRP水平。血清平均浓度为36.3 pmol/l±6.2（标准差），血清水平与年龄和性别无关。通过凝胶色谱法，随后使用合成α-和β-CGRP作为标准对正常血清提取物进行HPLC分析，证实了该分析方法的特异性。
