Wageningen University, The Netherlands.
Appl Microbiol Biotechnol. 2010 Sep;88(2):595-603. doi: 10.1007/s00253-010-2788-8. Epub 2010 Aug 3.
A mesophilic bacterium, strain An4, was isolated from an underground gas storage reservoir with methanol as substrate and perchlorate as electron acceptor. Cells were Gram-negative, spore-forming, straight to curved rods, 0.5-0.8 microm in diameter, and 2-8 microm in length, growing as single cells or in pairs. The cells grew optimally at 37 degrees C, and the pH optimum was around 7. Strain An4 converted various alcohols, organic acids, fructose, acetoin, and H(2)/CO(2) to acetate, usually as the only product. Succinate was decarboxylated to propionate. The isolate was able to respire with (per)chlorate, nitrate, and CO(2). The G+C content of the DNA was 42.6 mol%. Based on the 16S rRNA gene sequence analysis, strain An4 was most closely related to Sporomusa ovata (98% similarity). The bacterium reduced perchlorate and chlorate completely to chloride. Key enzymes, perchlorate reductase and chlorite dismutase, were detected in cell-free extracts.
一株嗜中温细菌，菌株 An4，从一个以甲醇为底物，高氯酸盐为电子受体的地下储气库中分离得到。细胞呈革兰氏阴性，产芽孢，直或弯杆状，直径 0.5-0.8 微米，长 2-8 微米，单个或成对生长。细胞在 37°C 下生长最佳，pH 最佳约为 7。菌株 An4 能将各种醇类、有机酸、果糖、乙酰醇和 H(2)/CO(2)转化为乙酸盐，通常为唯一产物。琥珀酸盐脱羧生成丙酸盐。该分离株能够以（高）氯酸盐、硝酸盐和 CO(2)进行呼吸。DNA 的 G+C 含量为 42.6 mol%。基于 16S rRNA 基因序列分析，菌株 An4 与 Sporomusa ovata 最为密切相关（相似度为 98%）。该细菌能将高氯酸盐和氯酸盐完全还原为氯离子。细胞提取物中检测到了过氯酸盐还原酶和亚氯酸盐歧化酶等关键酶。
