The Angstrom Laboratory, Department of Engineering Sciences, Uppsala University, Uppsala, Sweden.
J Mater Sci Mater Med. 2010 Oct;21(10):2743-9. doi: 10.1007/s10856-010-4137-y. Epub 2010 Aug 1.
Previous studies have shown that crystalline titanium oxide is in vitro bioactive and that there are differences in the HA formation mechanism depending on the crystalline direction of the titanium oxide surface. In the present study, the early adsorption of calcium and phosphate ions on three different surface directions of the single-crystal rutile TiO(2) substrate has been investigated. A crucial step in the nucleation of HA is believed to be the adsorption of Ca(2+) and PO(4)(3-) from phosphate buffer solutions. The (001), (100) and (110) single crystalline rutile surfaces were soaked in phosphate buffer saline solution for 10 min, 1 h and 24 h at 37°C. The surfaces were then analyzed using time-of-flight secondary ion mass spectrometry (TOF-SIMS) and X-ray photoelectron spectroscopy (XPS). The results show that the adsorption of Ca(2+) and PO(4)(3-) is faster on the (001) and (100) surfaces than on the (110) surface. This study also shows that TOF-SIMS can be used as a tool to better understand the adsorption of calcium and phosphate ions and the growth mechanism of HA. This knowledge could be used to tailor new bioactive surfaces for better biological reaction.
先前的研究表明，结晶氧化钛在体外具有生物活性，并且氧化钛表面的结晶方向的不同会导致 HA 形成机制的差异。在本研究中，研究了单晶金红石 TiO(2)基底的三个不同表面方向上早期钙和磷酸盐离子的吸附。人们认为，HA 成核的关键步骤是从磷酸盐缓冲溶液中吸附 Ca(2+)和 PO(4)(3-)。将（001）、（100）和（110）单晶金红石表面在 37°C 下分别浸泡在磷酸盐缓冲盐溶液中 10 分钟、1 小时和 24 小时。然后使用飞行时间二次离子质谱（TOF-SIMS）和 X 射线光电子能谱（XPS）对表面进行分析。结果表明，（001）和（100）表面上 Ca(2+)和 PO(4)(3-)的吸附速度比（110）表面快。本研究还表明，TOF-SIMS 可用作工具来更好地理解钙和磷酸盐离子的吸附以及 HA 的生长机制。这些知识可用于定制新的生物活性表面以获得更好的生物反应。
