Belu Anna M, Graham Daniel J, Castner David G
Department of Chemical Engineering, National ESCA for Biomedical Problems, University of Washington, 98195-351750, Seattle, WA, USA.
Biomaterials. 2003 Sep;24(21):3635-53. doi: 10.1016/s0142-9612(03)00159-5.
The surface of a biomaterial plays a critical role in the success of an implant. Much effort is currently being focused on controlling the chemistry at biomaterial surfaces to ensure favorable results in vivo. The successful tailoring of the surface chemistry will require a detailed surface characterization to verify that the desired changes have been made. This will include the ability to determine the composition, structure, orientation, and spatial distribution, of the molecules and chemical structures on the surface. TOF-SIMS is a powerful surface characterization technique that is able to address these requirements through both spectral analysis and direct chemical state imaging. The flexibility of the TOF-SIMS technique, and the wealth of data produced have generated much interest in its use for biomaterial characterization. This review discusses the strengths, weaknesses, and challenges of static TOF-SIMS for biomaterial surface characterization. First the basic principles of TOF-SIMS are introduced, giving an overview of the technique. Next, sample type, and other sample considerations are discussed. Then data interpretation is overviewed using examples from both spectral and imaging data. Finally, quantitative SIMS analysis is discussed and an outlook for TOF-SIMS analysis of biomaterials will be given.
生物材料的表面在植入物的成功应用中起着关键作用。目前，人们正致力于控制生物材料表面的化学性质，以确保在体内获得良好的效果。成功定制表面化学性质需要进行详细的表面表征，以验证是否已实现所需的变化。这将包括确定表面分子和化学结构的组成、结构、取向和空间分布的能力。飞行时间二次离子质谱（TOF-SIMS）是一种强大的表面表征技术，能够通过光谱分析和直接化学态成像来满足这些要求。TOF-SIMS技术的灵活性以及所产生的大量数据，使其在生物材料表征中的应用备受关注。本文综述了静态TOF-SIMS在生物材料表面表征方面的优势、劣势和挑战。首先介绍了TOF-SIMS的基本原理，概述了该技术。接下来讨论了样品类型和其他样品相关的注意事项。然后通过光谱和成像数据的示例对数据解释进行了概述。最后讨论了定量SIMS分析，并对生物材料的TOF-SIMS分析进行了展望。
