Department of Pediatrics, Chi-Mei Foundation Hospital, Tainan, Taiwan.
Obes Surg. 2011 Jul;21(7):935-40. doi: 10.1007/s11695-010-0241-9.
Receptor-interacting protein 140 (RIP140) is a corepressor for nuclear receptors with an important role in the inhibition of energy expenditure. Postmenopausal women have increased white adipose tissue (WAT), and excessive accumulation of adipose tissue (obesity) implies a health risk. The aim of the present work was to investigate the time course of RIP140 expression in WAT during the development of ovariectomy (OVX)-induced obesity in rats.
OVX was performed in female Sprague-Dawley (SD) rats 8 weeks old. Body weight and food intake were determined once a week. WAT of sham-operated, OVX and OVX plus 17β-estradiol therapy (OVX/E2) female SD rats was weighed and used to analyse RIP140 and uncoupling protein 1 (UCP-1) expression by Western blot.
Food intake and body weight were significantly increased during the 2-8 weeks after OVX. Even though body weight increased until killing, food intake progressively decreased from 9 to 16 weeks after OVX in rats. Meanwhile, increased WAT mass and decreased RIP140 expression in WAT were observed in OVX rats. In contrast, the expression of UCP-1, a key target gene of RIP140, in WAT of OVX rats was significantly higher than in sham-operated rats. All of these alterations caused by OVX were mostly reversed by the replacement of 17β-estradiol.
The down-regulation of RIP140 in WAT may play a compensatory role in OVX-induced obesity in rat.
受体相互作用蛋白 140（RIP140）是核受体的核心抑制剂，在抑制能量消耗方面发挥着重要作用。绝经后妇女的白色脂肪组织（WAT）增加，而脂肪组织过度堆积（肥胖）意味着健康风险。本研究旨在探讨 RIP140 在去卵巢（OVX）诱导肥胖大鼠 WAT 中的表达时间过程。
8 周龄雌性 Sprague-Dawley（SD）大鼠行 OVX。每周测定一次体重和摄食量。称重 sham 手术、OVX 和 OVX 加 17β-雌二醇治疗（OVX/E2）的雌性 SD 大鼠的 WAT，并通过 Western blot 分析 RIP140 和解偶联蛋白 1（UCP-1）的表达。
OVX 后 2-8 周，摄食量和体重明显增加。尽管体重一直增加到处死，但 OVX 后 9-16 周大鼠的摄食量逐渐减少。与此同时，OVX 大鼠的 WAT 质量增加，WAT 中的 RIP140 表达减少。相反，OVX 大鼠 WAT 中 RIP140 的关键靶基因 UCP-1 的表达明显高于 sham 手术大鼠。OVX 引起的所有这些改变大多被 17β-雌二醇替代所逆转。
WAT 中 RIP140 的下调可能在大鼠 OVX 诱导的肥胖中发挥代偿作用。
