Bijvoet Center for Biomolecular Research, Utrecht University, Padualaan 8, 3584 CH, Utrecht, The Netherlands.
J Biomol NMR. 1997 Apr;9(3):313-6. doi: 10.1023/A:1018687127330.
A new method to selectively detect the ring resonances of the aromatic residues in 15N-labelled proteins is presented. The experiment consists of a 2D 1H TOCSY sequence withremoval of the amide signals via 15N-filtering. Experiments are acquired in the absence andpresence of water inversion; combining the two spectra allows selective observation of thetyrosine ring resonances and enables the identification of their delta andepsilon ring protons. The experiment is demonstrated on a 15N-labelled sample of Photoactive Yellow Protein and isshown to give good selectivity for tyrosine ring resonances under a wide range oftemperatures and pH values.
本文提出了一种新的方法，用于选择性地检测 15N 标记蛋白质中芳香族残基的环共振。该实验由二维 1H TOCSY 序列组成，通过 15N 过滤去除酰胺信号。实验在没有和存在水反转的情况下进行；将两个光谱结合起来，可以选择性地观察酪氨酸环共振，并能够识别它们的 δ 和 ε 环质子。该实验在光活性黄蛋白的 15N 标记样品上进行，并证明在较宽的温度和 pH 值范围内对酪氨酸环共振具有良好的选择性。
